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BIBTEX RIS

Differential expression of miRNAs related to angiogenesis and adipogenesis in subcutaneous fat of obese and nonobese women

2018; Springer Nature; Volume: 46; Issue: 1 Linguagem: Inglês

10.1007/s11033-018-4553-5

ISSN

1573-4978

Autores

Aline Gasparotto, Diego Olschowsky Borges, Marina G. M. Sassi, Adriana Rosa Milani, Darwin L. Rech, Marcia Terres, Pedro Bins Ely, Maurício Jacques Ramos, Nelson Guardiola Meihnardt, Vanessa S. Mattevi,

Tópico(s)

Cancer-related molecular mechanisms research

Resumo

To disclose the mechanisms surrounding obesity, we selected microRNAs (miRNAs) that target genes involved in adipogenesis, angiogenesis, and inflammation and compared their expression levels in the subcutaneous adipose tissue of 40 obese and nonobese women. Mature miRNAs were extracted from subcutaneous adipose tissue samples that were collected during surgery and quantified by real-time polymerase chain reaction. miR-16 was overexpressed in the nonobese group (n-expression ratio = − 151.1; P < 0.001). Furthermore, the expression levels of two other miRNAs were significantly correlated with waist circumference in nonobese women (miR-27b, r = 0.453; P = 0.027 and miR-424-5p, r = 0.502, P = 0.014). Central and total subcutaneous adipose tissue thicknesses were correlated with miR-424-5p levels (r = 0.506, P = 0.034 and r = 0.475, P = 0.046, respectively) in the nonobese group. In the obese group, miR-424-5p expression was correlated with body mass index (r = 0.582, P = 0.018). miR-16 and miR-424 have shown correlations with body-fat-mass-related parameters. Because these miRNAs have vascular endothelial growth factor (VEGF) and its receptors as target genes, they may be involved in the alterations of angiogenesis observed in obesity. In addition, higher levels of miR-27 and miR-424 were correlated with higher fat depot measurements in nonobese women. These results highlight the importance of miRNA expression in subcutaneous adipose tissue and encourage further investigation of miRNAs as prognostic markers.

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