Produção Nacional
Revisado por pares
Determination of phenolic compounds, in vitro antioxidant activity and characterization of secondary metabolites in different parts of Passiflora cincinnata by HPLC-DAD-MS/MS analysis
2018; Taylor & Francis; Volume: 34; Issue: 7 Linguagem: Inglês
10.1080/14786419.2018.1548445
ISSN1478-6427
AutoresAna Ediléia Barbosa Pereira Leal, Ana Paula de Oliveira, Raira Feitosa dos Santos, Juliana Mikaelly Dias Soares, Érica Martins de Lavor, Michelle Cruz Pontes, Julianeli Tolentino de Lima, Alan Diego da Conceição Santos, José Carlos Tomaz, Gibson Gomes de Oliveira, Fausto Carnevale Neto, Norberto Peporine Lopes, Larissa Araújo Rolim, Jackson Roberto Guedes da Silva Almeida,
Tópico(s)Phytochemistry and Biological Activities
ResumoEthanol extracts of different parts of Passiflora cincinnata were obtained by maceration. The total phenolic and flavonoid contents were evaluated. The antioxidant activities were determined by β-carotene-linoleic acid bleaching test, 2,2-diphenyl-1-picrylhydrazil (DPPH), and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging. The crude ethanol stem extract showed the highest amount of total polyphenols (45.53 mg gallic acid equivalent/g) while the highest total flavonoid contents (1.42 mg of quercetin equivalent/g) were observed in the leaf extract. The lowest IC50 (25.65 μg/ml) by the DPPH method was observed for the stem extract. The ABTS method showed a significant antioxidant activity for all investigated extracts. The secondary metabolite composition of ethanol extracts was assessed by HPLC-DAD-MS/MS analysis, leading to the identification of fourteen secondary metabolites in P. cincinnata extracts. These results showed the potentiality of this species as a source of phenolic compounds and antioxidants.
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