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Comprehensive analysis of kinase-oriented phospho-signalling pathways

2018; Oxford University Press; Volume: 165; Issue: 4 Linguagem: Inglês

10.1093/jb/mvy115

1756-2651

Mutsuki Amano, Tomoki Nishioka, Daisuke Tsuboi, Keisuke Kuroda, Yasuhiro Funahashi, Yukie Yamahashi, Kozo Kaibuchi,

Microtubule and mitosis dynamics

Accumulating information on eukaryotic protein phosphorylation implies a large and complicated phospho-signalling network in various cellular processes. Although a large number of protein phosphorylation sites have been detected, their physiological consequences and the linkage between each phosphorylation site and the responsible protein kinase remain largely unexplored. To understand kinase-oriented phospho-signalling pathways, we have developed novel substrate screening technologies. In this review, we described the in vitro and in vivo screening methods named kinase-interacting substrate screening analysis and kinase-oriented substrate screening analysis, respectively.