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Prevalência de Ehrlichia canis pela Nested-PCR, correlação com a presença de mórula e trombocitopenia em cães atendidos no Hospital Veterinário da Universidade Federal do Espírito Santo

2015; Sociedade de Medicina Veterinária do Estado do Rio de Janeiro; Volume: 37; Issue: 1 Linguagem: Inglês

2527-2179

Mara Rúbia Rocha Pereira Sales, Mariana Drummond Costa Ignacchiti, Aguinaldo Francisco Mendes, Weslem Garcia Suhett, Lenir Cardoso Porfírio, Mozart Marins, Karina Preising Aptekmann, Olavo dos Santos Pereira Júnior,

Viral Infections and Vectors

ABSTRACT. Sales M.R.R.P., Ignacchiti M.D.C., Mendes Junior A.F., Suhett W.G., Porfirio L.C., Marins M., Aptekmann K.P. & Pereira Junior O.S. [Prevalence of Ehrlichia canis using the nested-PCR, correlation with the presence of morulae and thrombocytopenia in dogs treated in Veterinary Hospital of the Federal University of Espirito Santo.] Prevalencia de Ehrlichia canis pela Nested- -PCR, correlacao com a presenca de morula e trombocitopenia em caes atendidos no Hospital Veterinario da Universidade Federal do Espirito Santo. Revista Brasileira de Medicina Veterinaria, 37(1):47-51, 2015. Centro de Ciencias Agrarias, Universidade Federal do Espirito Santo, Rua Projetada s/no, Caixa Postal 25, Pontal, Marataizes, ES 29349-000, Brasil. E-mail: mararrps@yahoo.com.br Ehrlichia canis, is the primary etiologic agent of canine monocytic ehrlichiosis. The disease is mainly transmitted by the brown dog ticks Rhipicephalus sanguineus in different endemic regions of Brazil. The purpose of this study was determinated using the Nested Polymerase Chain Reaction (nested-PCR) the prevalence of Ehrlichia canis in 85 dogs, regardless of race, age, sex or health status, treated at the Veterinary Hospital of Federal University of Espirito Santo, in Alegre-ES and evaluate its correlation with the presence of morulae and thrombocytopenia. It was observed that 1.17% of the samples were positive by blood smear, for the presence of morulae. However, the nested-PCR showed 5.88% positivity of samples. And 17.64% samples showed thrombocytopenia. By analyzing all the techniques, it was concluded that the introduction of diagnostic techniques such as nested-PCR is an important method for aid in early diagnosis of pathologies.