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First report of Corynespora cassiicola causing leaf spot on Solanum americanum in Brazil

2019; Springer Science+Business Media; Volume: 101; Issue: 3 Linguagem: Inglês

10.1007/s42161-018-00216-z

2239-7264

Wagner Vicente Pereira, Louise Larissa May De Mio,

Plant-Microbe Interactions and Immunity

Weed .Tomato .Solanum americanum Solanum americanum Mill. is one of the most frequently and widely distributed weeds in tomato areas in Brazil.The competition for nutrients and allelopathy effect exerted by S. americanum on tomato plants has caused intense damage to tomato production in Brazil.In addition, S. americanum can serve as a host of phytopathogens.In Brazil, Corynespora cassiicola (Berk.& M.A. Curtis) C.T. Wei causes major diseases in tomato, soybean and coffee tree.In January 2018, leaf spots were observed in S. americanum in four hectares of tomato crop in São Paulo state, Brazil.S. americanum plants were distributed throughout the area and around 50% of the leaves were infected.The leaves had dark brown circular or irregular spots, with concentric rings and yellow halo.Isolations were performed by transferring little samples (0.5 cm 2 ) of superficially disinfected plant tissues from symptomatic leaves onto Petri plates containing PDA (potato dextrose agar) and cultured at 25 °C with 12-h photoperiod during 14 days.From 10 samples used in the isolation, 80% provided the development of colonies in the PDA medium.The resulting colonies were deposited in the culture collection of the plant pathology laboratory of Federal University of Paraná.Those colonies were dark gray, circular, with dense and velvety mycelial growth.The conidia (n = 50) were straight to curved, cylindrical, 49-151 × 8-11 μm, brown, with 3 to 14 pseudosepta.The conidiophores (n = 50) were isolated, septate, brown, cylindrical, 98-211 × 8-10 μm.These characteristics are similar to those described for C. cassiicola (Ellis and Holliday 1971).To confirm the morphological identification, the internal transcribed spacer (ITS) region was amplified using the pair of primers ITS1 *