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DNA methyltransferase BmDnmt1 and BmDnmt2 in silkworm ( Bombyx mori ) and the regulation of silkworm embryonic development

2018; Wiley; Volume: 100; Issue: 3 Linguagem: Inglês

10.1002/arch.21529

1520-6327

Bing Li, Pei Hu, Shang-zhi Zhang, Shahzad Toufeeq, Jie Wang, Kang Zhao, Xin Xu, Jia Xu, Shoujun Huang,

Cancer-related gene regulation

Abstract DNA methylation is one of the most widespread epigenetic marks and has been linked to insect development, especially influencing embryonic development. However, the regulation of DNA methylation in silkworm embryonic development and diapause remain to investigate. In this study, reverse‐transcription quantitative polymerase chain reaction was performed to identify the expression level of Bombyx mori DNA methyltransferases (BmDNMTs) 1 and 2 ( BmDnmt1 and BmDnmt2 ) in different tissues, different embryonic developmental stages, and different strains of the silkworm. The results showed that BmDNMT s were the most highly expressed during embryonic development, especially at early embryonic stages. In particular, the expression of BmDNMTs was significantly upregulated in diapause‐terminated eggs by HCl treatment. Moreover, tissue distribution showed that BmDnmt2 was highly expressed in testis and ovary, and BmDnmt1 was highly expressed in testis. This study contributes to understanding the correlation of DNA methylation occurs with embryogenesis and gametogenesis in insect, meanwhile, it provides a research orientation to further analyze the role of DNA methylation in diapause initiation and termination in insect embryonic development.