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Prospective evaluation of diagnostic tools for respiratory viruses in children and adults

2019; Elsevier BV; Volume: 266; Linguagem: Inglês

10.1016/j.jviromet.2019.01.006

1879-0984

Laurent Busson, Magali Bartiaux, Safa Ben Brahim, Déborah Konopnicki, Nicolás Dauby, M Gérard, P. De Backer, Kristien Van Vaerenbergh, Bhavna Mahadeb, Marc De Foor, Magali Wautier, Olivier Vandenberg, Pierre Mols, Jaclyn Levy, Marie Hallin,

Pneumonia and Respiratory Infections

To compare the performances of molecular and non-molecular tests to diagnose respiratory viral infections and to evaluate the pros and contras of each technique. Two hundred ninety-nine respiratory samples were prospectively explored using multiplex molecular techniques (FilmArray Respiratory Panel, Clart Pneumovir), immunological techniques (direct fluorescent assay, lateral flow chromatography) and cell cultures. Molecular techniques permitted the recovery of up to 50% more respiratory pathogens in comparison to non-molecular methods. FilmArray detected at least 30% more pathogens than Clart Pneumovir which could be explained by the differences in their technical designs. The turnaround time under 2 hours for the FilmArray permitted delivery of results when patients were still in the emergency room.