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Viabilidade espermática de sêmen congelado de suínos da raça Piau avaliada pelo teste de termorresistência

2014; Sociedade de Medicina Veterinária do Estado do Rio de Janeiro; Volume: 36; Issue: 2 Linguagem: Inglês

2527-2179

Maurício Hoshino da Costa Barros, Hugo Hideki Shiomi, Lincoln da Silva Amorim, Simone Eliza Facioni Guimarães, Paulo Sávio Lopes, Jeanne Broch Siqueira, Rogério Oliveira Pinho, José Domingos Guimarães,

Reproductive Biology and Fertility

ABSTRACT. Barros M.H.C., Shiomi H.H., Amorim L.S., Guimaraes S.E.F., Lopes P.S., Siqueira J.B., Pinho R.O. & Guimaraes J.D. [Spermatic Viability of Cryopreserved Semen of Piau swine breed analyzed by Thermo Resistant Test.] Viabilidade espermatica de semen congelado de suinos da raca Piau avaliada pelo Teste de Termorresistencia. Revista Brasileira de Medicina Veterinaria 36(2):131-136, 2014. Departamento de Veterinaria, Universidade Federal de Vicosa, Av. Peter Henry Rolfs, s/n, Vicosa, MG 36571-000, Brasil. E-mail: hugoshiomi@hotmail.com The objective of this study was to verify three protocols of semen cryopreservation on spermatic viability after thawing from Piau swine breed (Sus scrofa), by thermo resistant test (TTR). Twenty two ejaculates from 5 Piau mature boars were collected by glove hand technique. To freezing, the ejaculates was split and submitted to three protocols (P): (P1) freezing method proposed by Furst et al. (2005), altered by diluent media, (P2) freezing method proposed by Furst et al. (2005), altered by cooled curve; and (P3) freezing method proposed by Ohata et al. (2001). After thawing, semen was submitted to TTR, been incubated at 37o C by 2 hours, motility sperm (MOT) and vigor (VIG) was analyzed at 30 minutes of interval. MOT and VIG after thawing was 20.9±12.4, 29.5±10.9 and 49.5±12.1%; 2.5±0.5, 2.9±0.4 and 3.4±0.4, respectively to P1, P2 and P3. The TTR results show gradually decrease of motility and vigor along 2 hours of test procedure utilized, with best average to protocol 3 at all time of analyze. The protocol 3 tested by Piau boars shows highest values in cellular semen cryopreservation.