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Ontogeny of lymphoid organs in meagre (Argyrosomus regius) reared at different temperatures

2018; Frontiers Media; Volume: 5; Linguagem: Inglês

10.3389/conf.fmars.2018.06.00049

2296-7745

Damiana Pires, Ana Gamboa, Sara Castanho, Anaísa Silva, Teresa Baptista, Pedro Pousão‐Ferreira, Florbela Soares,

Reproductive biology and impacts on aquatic species

Event Abstract Back to Event Ontogeny of lymphoid organs in meagre (Argyrosomus regius) reared at different temperatures Damiana M. Pires1*, Ana Gamboa2, Sara Castanho2, Anaísa Silva2, Teresa M. Baptista1, Pedro Pousão-Ferreira2 and Florbela Soares2 1 Escola Superior de Turismo e Tecnologia do Mar, Politécnico de Leiria, MARE – Marine and Environmental Sciences Centre, Portugal 2 Estação Piloto de Piscicultura de Olhão, Instituto Português do Mar e Atmosfera, Portugal Argyrosomus regius is a new specie in aquaculture with limited knowledge of the timing of appearance and development of its immune system during ontogeny development, important to prevent disease outbreaks. The first step is to determine the appearance of the lymphoid organs, so that prophylactic measures can be applied, thus individuals acquire resistance instead of tolerance. This study evaluated the appearance of lymphoid organs in meagre larvae reared at two different temperatures (15±0.7 and 18±0.5°C). The larvae were obtained from a natural spawn of F1 meagre broodstock maintained in the IPMA’s (Portuguese Institute for the Sea and Atmosphere) Aquaculture research station (EPPO). The larval rearing was performed in cylindroconical tanks, in duplicate with an initial density of 26 larvae L-1. The photoperiod was 14h-10h light-dark and the water renewal started at 15%.h-1 increasing up to 40%.h-1 with larval growth and development in an open system. The green water method was applied, from 3 days’ post hatching (DPH) to 10 DPH, the larvae were fed with rotifers (Brachionus spp.). Artemia was provided since 8 DPH and the inert feed was introduced at 9 DPH. Both, rotifers and Artemia were enriched with RedPepper ® (Bernaqua TM) before provided. Samples were collected daily for total length (mm), dry weight (mg) and histology. After 20 DPH , larvae presented a higher length (6.67±0.56 mm) in the 18°C treatment compared with the larvae from 15°C treatment (4.77±0.38 mm). Further, it will be verified if the differences in growth were followed by an earlier lymphatic organs appearance, in order to define the prophylactic measures to be applied in larvae rearing protocols. Acknowledgements This study had the support of project DIVERSIAQUA (Mar2020 16-02-01-FMP-0066). Keywords: Aquaculture, Marine fish larvae, Immune System, Head Kidney, Thymus, Spleen Conference: IMMR'18 | International Meeting on Marine Research 2018, Peniche, Portugal, 5 Jul - 6 Jul, 2018. Presentation Type: Poster Presentation Topic: Aquaculture Citation: Pires DM, Gamboa A, Castanho S, Silva A, Baptista TM, Pousão-Ferreira P and Soares F (2019). Ontogeny of lymphoid organs in meagre (Argyrosomus regius) reared at different temperatures. Front. Mar. Sci. Conference Abstract: IMMR'18 | International Meeting on Marine Research 2018. doi: 10.3389/conf.FMARS.2018.06.00049 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 May 2018; Published Online: 07 Jan 2019. * Correspondence: Mrs. Damiana M Pires, Escola Superior de Turismo e Tecnologia do Mar, Politécnico de Leiria, MARE – Marine and Environmental Sciences Centre, Peniche, Portugal, dami.pires@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Damiana M Pires Ana Gamboa Sara Castanho Anaísa Silva Teresa M Baptista Pedro Pousão-Ferreira Florbela Soares Google Damiana M Pires Ana Gamboa Sara Castanho Anaísa Silva Teresa M Baptista Pedro Pousão-Ferreira Florbela Soares Google Scholar Damiana M Pires Ana Gamboa Sara Castanho Anaísa Silva Teresa M Baptista Pedro Pousão-Ferreira Florbela Soares PubMed Damiana M Pires Ana Gamboa Sara Castanho Anaísa Silva Teresa M Baptista Pedro Pousão-Ferreira Florbela Soares Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.