Produção Nacional
Revisado por pares
First Report of Phytophthora palmivora Causing Black Pod on a Herrania sp. in Brazil
2019; American Phytopathological Society; Volume: 103; Issue: 6 Linguagem: Inglês
10.1094/pdis-07-18-1287-pdn
ISSN1943-7692
AutoresJennifer Decloquement, Roberto Ramos‐Sobrinho, Dahyana Britto, Ailton Reis, Jaime Honorato-Júnior, Danilo Batista Pinho, Jean‐Philippe Marelli,
Tópico(s)Plant Pathogens and Fungal Diseases
ResumoHomePlant DiseaseVol. 103, No. 6First Report of Phytophthora palmivora Causing Black Pod on a Herrania sp. in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Phytophthora palmivora Causing Black Pod on a Herrania sp. in BrazilJ. Decloquement, R. Ramos-Sobrinho, D. S. Britto, A. Reis, J. Honorato-Júnior, D. B. Pinho, and J. P. MarelliJ. DecloquementDepartamento de Fitopatologia, Universidade de Brasília, Brasília, DF, Brazil; Search for more papers by this author, R. Ramos-Sobrinho†Corresponding author: R. Ramos-Sobrinho; E-mail Address: cbrobertorsb@hotmail.comhttp://orcid.org/0000-0002-7280-3916Departamento de Fitopatologia, Universidade de Brasília, Brasília, DF, Brazil; Search for more papers by this author, D. S. BrittoMars Center for Cocoa Science, Barro Preto, BA, Brazil; Search for more papers by this author, A. Reishttp://orcid.org/0000-0002-5705-3002Embrapa, Centro Nacional de Pesquisa de Hortaliças, Brasília, DF, Brazil; Search for more papers by this author, J. Honorato-JúniorCentro Multidisciplinar do Campus de Barra, Universidade Federal do Oeste da Bahia, Barra, BA, Brazil; and Search for more papers by this author, D. B. Pinhohttp://orcid.org/0000-0003-2624-302XDepartamento de Fitopatologia, Universidade de Brasília, Brasília, DF, Brazil; Search for more papers by this author, and J. P. MarelliMARS Inc., c/o USDA-ARS, Subtropical Horticulture Research Station, Miami, FL, U.S.A.Search for more papers by this authorAffiliationsAuthors and Affiliations J. Decloquement1 R. Ramos-Sobrinho1 † D. S. Britto2 A. Reis3 J. Honorato-Júnior4 D. B. Pinho1 J. P. Marelli5 1Departamento de Fitopatologia, Universidade de Brasília, Brasília, DF, Brazil; 2Mars Center for Cocoa Science, Barro Preto, BA, Brazil; 3Embrapa, Centro Nacional de Pesquisa de Hortaliças, Brasília, DF, Brazil; 4Centro Multidisciplinar do Campus de Barra, Universidade Federal do Oeste da Bahia, Barra, BA, Brazil; and 5MARS Inc., c/o USDA-ARS, Subtropical Horticulture Research Station, Miami, FL, U.S.A. Published Online:21 Mar 2019https://doi.org/10.1094/PDIS-07-18-1287-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat During inspections in a germplasm collection in the municipality of Barro Preto, Bahia State, Brazil, it was observed that about 30% of the pods of a Herrania sp. (Malvaceae) genotype showed black pod rot in 2017. Symptoms initially appeared as brown, circular lesions that quickly enlarged at high humidity, and a white mycelium covered the diseased pod surface. The putative pathogen was isolated from six symptomatic pods on 2% water agar containing ampicillin, rifampicin, nystatin, and pentachloronitrobenzene. A hyphal-tip culture from a morphologically representative isolate was obtained on carrot agar (CA) and deposited in the Coleção de Fungos Fitopatogênicos of the Universidade de Brasília (accession no. CCUB1089). The colony on CA was white and slightly cottony with sparse aerial mycelium. Sporangia (n = 50) were ellipsoid, deciduous, 32.5 to 55.5 µm long, and 25.5 to 39 μm wide, and papillae were 6.0 to 7.0 µm thick. Globose, terminal chlamydospores measuring 16.5 to 39.5 µm in diameter were formed abundantly on CA. The specimen was identified as mating type A1 by pairing with A1 and A2 tester strains of Phytophthora capsici and P. nicotianae. Partial nucleotide sequences of the internal transcribed spacer (ITS), β-tubulin (βt), elongation factor (EF), heat shock protein 90 (HSP90), and cytochrome oxidase subunit 2 (coxII) genomic regions were obtained and deposited in GenBank (accession nos. MH807778 to MH807782). In the Phytophthora database (Park et al. 2008), the ITS, coxII, βt, EF, and HSP90 sequences matched P. palmivora (isolate PD_00022) with 100.0, 100.0, 99.5, 99.8, and 98.9% identities (accession nos. HQ261635, GU222099, EU080339, EU080340, and EU080342, respectively). Additionally, our isolate clustered with other P. palmivora isolates in a Bayesian phylogenetic tree based on concatenated sequences (ITS, coxII, βt, EF, and HSP90) (study S23112 deposited in TreeBASE). Thus, based on morphological and molecular data, the pathogen was identified as P. palmivora. To confirm pathogenicity, mycelial plugs (5 mm) from a 7-day-old culture (grown on CA at 25°C) were placed onto three nonwounded pods of Herrania sp., Theobroma cacao (clone CCN51), T. grandiflorum, T. bicolor, T. speciosum, and T. subincanum. On control pods, CA discs were used. Pods were kept for 5 days in a humid chamber at 25°C. Inoculated pods showed symptoms similar to those observed in the field, and P. palmivora was successfully reisolated from all symptomatic pods. Control pods were symptomless. P. palmivora has been previously reported on Herrania sp. only in West Africa (Erwin and Ribeiro 1996). In Brazil, this pathogen is infecting Annona squamosa, Carica papaya, Citrus sp., Cocos nucifera, Ficus carica, Hevea brasiliensis, Solanum lycopersicum, Piper nigrum, T. cacao, and T. grandiflorum (dos Santos et al. 2017; Farr and Rossman 2018; Zentmyer et al. 1973). The pathogenicity of P. palmivora on T. bicolor, T. speciosum, and T. subincanum (and the absence of reports on these hosts) reinforces that this pathogen can infect species closely related to cacao. This is the first report of P. palmivora causing black pod on a Herrania sp. in Brazil.The author(s) declare no conflict of interest.References:dos Santos, A. F., et al. 2017. Plant Dis. 101:1331. https://doi.org/10.1094/PDIS-08-16-1143-PDN Link, ISI, Google ScholarErwin, D. E., and Ribeiro, O. R. 1996. Phytophthora Diseases Worldwide. American Phytopathological Society, St. Paul, MN. Google ScholarFarr, D. F., and Rossman, A. Y. 2018. Fungal Databases, Syst. Mycol. Microbiol. Lab., ARS, USDA. Retrieved 20 July 2018 from https://nt.ars-grin.gov/fungaldatabases/. Google ScholarPark, J., et al. 2008. Plant Dis. 92:966. https://doi.org/10.1094/PDIS-92-6-0966 Link, ISI, Google ScholarZentmyer, G. A., et al. 1973. Phytopathology 63:663. https://doi.org/10.1094/Phyto-63-663 Crossref, ISI, Google ScholarThe author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 103, No. 6 June 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionGreen mottle mosaic and leaf deformation symptoms on watermelon (Sui, Li, Shamimuzzaman, Wu, and Ling). Photo credit: K.-S. Ling. Postharvest rot on cucumber caused by Ceratocystis fimbriata (Li, Xu, Zhang, Song, Xie, Sun, and Huang). Photo credit: H. Song. Metrics Article History Issue Date: 6 Jun 2019Published: 21 Mar 2019First Look: 25 Jan 2019Accepted: 16 Jan 2019 Page: 1435 Information© 2019 The American Phytopathological SocietyThe author(s) declare no conflict of interest.Cited byPhytophthora palmivora (coconut budrot)CABI Compendium, Vol. CABI CompendiumPhytophthora theobromicola sp. nov.: A New Species Causing Black Pod Disease on Cacao in Brazil15 March 2021 | Frontiers in Microbiology, Vol. 12Morphologic, molecular, and pathogenic characterization of Phytophthora palmivora isolates causing flower rot on azalea26 May 2020 | Brazilian Journal of Microbiology, Vol. 51, No. 4
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