First Report of TYLCV-IS141, a Tomato Yellow Leaf Curl Virus Recombinant Infecting Tomato Plants Carrying the Ty-1 Resistance Gene in Sardinia (Italy)
2019; American Phytopathological Society; Volume: 103; Issue: 6 Linguagem: Inglês
10.1094/pdis-09-18-1558-pdn
ISSN1943-7692
AutoresMartine Granier, L. Tomassoli, A. Manglli, M. Nannini, Michel Peterschmitt, Cica Urbino,
Tópico(s)Plant Disease Resistance and Genetics
ResumoHomePlant DiseaseVol. 103, No. 6First Report of TYLCV-IS141, a Tomato Yellow Leaf Curl Virus Recombinant Infecting Tomato Plants Carrying the Ty-1 Resistance Gene in Sardinia (Italy) PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of TYLCV-IS141, a Tomato Yellow Leaf Curl Virus Recombinant Infecting Tomato Plants Carrying the Ty-1 Resistance Gene in Sardinia (Italy)M. Granier, L. Tomassoli, A. Manglli, M. Nannini, M. Peterschmitt, and C. UrbinoM. GranierCIRAD, UMR BGPI, F-34398 Montpellier, France; , L. TomassoliConsiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di ricerca, Difesa e Certificazione CREA-DC, I00156 Rome, Italy; and , A. ManglliConsiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di ricerca, Difesa e Certificazione CREA-DC, I00156 Rome, Italy; and , M. NanniniAgris Sardegna, IT 09123 Cagliari, Italy, M. PeterschmittCIRAD, UMR BGPI, F-34398 Montpellier, France; , and C. Urbino†Corresponding author: C. Urbino; E-mail Address: cica.urbino@cirad.frhttp://orcid.org/0000-0002-6215-9389CIRAD, UMR BGPI, F-34398 Montpellier, France; AffiliationsAuthors and Affiliations M. Granier1 L. Tomassoli2 A. Manglli2 M. Nannini3 M. Peterschmitt1 C. Urbino1 † 1CIRAD, UMR BGPI, F-34398 Montpellier, France; 2Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di ricerca, Difesa e Certificazione CREA-DC, I00156 Rome, Italy; and 3Agris Sardegna, IT 09123 Cagliari, Italy Published Online:22 Mar 2019https://doi.org/10.1094/PDIS-09-18-1558-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Tomato yellow leaf curl virus (TYLCV), tomato yellow leaf curl Sardinia virus (TYLCSV), and interspecies recombinants derived from them were detected in tomato (Solanum lycopersicum) in Italy (Panno et al. 2018). Cultivars carrying the Ty-1 gene are a key component in the control of tomato yellow leaf curl disease (TYLCD); Ty-1 prevents symptom expression and reduces virus accumulation. During a survey in September 2016 in Sardinia (Italy), symptomatic infections typical of TYLCD were observed on tomato plants of the resistant cultivar Parioli, in commercial greenhouses of the province of Cagliari, suggesting infection with TYLCV/TYLCSV recombinants (Belabess et al. 2015; Panno et al. 2018) or betasatellite (Conflon et al. 2018), which were both associated with the break of Ty-1 resistance. Total DNA was extracted from leaf samples of three symptomatic and one nonsymptomatic plants collected in a single greenhouse. Using polymerase chain reaction (PCR) tests, all four samples were positive for the presence of the Ty-1 gene at the heterozygous state (Verlaan et al. 2011) but negative for the presence of betasatellite DNA (Briddon et al. 2002). According to multiplex PCR tests (Belabess et al. 2015), the nonsymptomatic sample was negative for the detection of TYLCV, TYLCSV, and TYLCV/TYLCSV recombinants; one symptomatic sample was positive for all three types of genomes, and the other two were positive for TYLCSV and TYLCV/TYLCSV recombinants. These latter two DNA extracts were used in rolling circle amplification (RCA). To select recombinant genomes for cloning, the RCA product was digested with SacI, because this restriction site is unique in TYLCV and absent in TYLCSV genomes. A 2.8-kb restricted fragment was cloned in the SacI-linearized plasmid pBC, and three clones, obtained from the two DNA extracts, were sequenced. The three sequenced genomes had 2,773 nucleotides (nt) and shared 99% identity to each other. Like TYLCV-IL-[IT:Sic23:16], a TYLCV/TYLCSV recombinant recently detected from Ty-1 resistant plants sampled in Sicily in 2016 (Panno et al. 2018), the Sardinian genomes were composed mostly of a TYLCV-IL sequence, except 124 nt (nt 16 to 139) between the origin or replication and the ATG codon of the V2 gene, which were derived from TYLCSV. The highest nt identity of the TYLCSV sequence was detected with a TYLCSV isolate from Sicily, accession no. Z28390 (92%), and that of the TYLCV sequence was detected with TYLCV-IL-[IT:Sic:04], DQ144621 (98%). Because the sequence identity of Sardinian and Sicilian recombinants was higher than 94% (97.6%), they were considered to belong to the same group of TYLCV-IL isolates previously named TYLCV-IS141 (Belabess et al. 2018). For the sake of clarity, we propose to give this name to all recombinants with the same recombination event. Thus, the sequence deposited to GenBank (MH817479) was called TYLCV-IS141 (TYLCV-IL-[IT:Sar IS141:16]). Because this recombinant was detected in coinfection with TYLCSV or TYLCV, it cannot be stated that it caused TYLCD symptoms on resistant plants. Moreover, these symptoms were not observed during the following year. However, TYLCV-IS141 recombinants were already detected from plants collected in 2012 and 2016 in Sicily (Belabess et al. 2015, 2018; Panno et al. 2018), and it was found to be positively selected in Ty-1 resistant plants (Belabess et al. 2018). Further samplings are needed to estimate the prevalence of TYLCV-IS141 recombinants in Sardinia and Sicily and to test their fitness in Ty-1 resistant plants.The author(s) declare no conflict of interest.References:Belabess, Z., et al., 2015. Virology 486:291. https://doi.org/10.1016/j.virol.2015.09.011 Google ScholarBelabess, Z., et al., 2018. Virus Res. 243:44. https://doi.org/10.1016/j.virusres.2017.10.002 Google ScholarBriddon, R. W., et al., 2002. Mol. Biotechnol. 20:315. https://doi.org/10.1385/mb:20:3:315 Google ScholarConflon, D., et al., 2018. Virus Res. 253:124. https://doi.org/10.1016/j.virusres.2018.06.003 Google ScholarPanno, S., et al., 2018. Arch. Virol. 163:795. Google ScholarVerlaan, M., et al., 2011. Plant J. 68:1093. https://doi.org/10.1111/j.1365-313X.2011.04762.x Google ScholarThe author(s) declare no conflict of interest.Funding: Funding was provided by Agence Nationale de la Recherche (ERANET-ARIMNet2 Call 2015).DetailsFiguresLiterature CitedRelated Vol. 103, No. 6 June 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionGreen mottle mosaic and leaf deformation symptoms on watermelon (Sui, Li, Shamimuzzaman, Wu, and Ling). Photo credit: K.-S. Ling. Postharvest rot on cucumber caused by Ceratocystis fimbriata (Li, Xu, Zhang, Song, Xie, Sun, and Huang). Photo credit: H. Song. Metrics Article History Issue Date: 6 Jun 2019Published: 22 Mar 2019First Look: 4 Feb 2019Accepted: 28 Jan 2019 Pages: 1437-1437 InformationThis article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 2019.FundingAgence Nationale de la RechercheGrant/Award Number: ERANET-ARIMNet2 Call 2015The author(s) declare no conflict of interest.Cited byBegomoviruses: what is the secret(s) of their success?Trends in Plant Science, Vol. 102The Role of Extensive Recombination in the Evolution of Geminiviruses3 January 2023An Effective Integrated Machine Learning Framework for Identifying Severity of Tomato Yellow Leaf Curl Virus and Their Experimental ValidationResearch, Vol. 6Invasive tomato yellow leaf curl virus recombinants challenge virus diagnosis and disease managementThe Global Dimension of Tomato Yellow Leaf Curl Disease: Current Status and Breeding Perspectives1 April 2021 | Microorganisms, Vol. 9, No. 4Metagenomics of Neotropical Single-Stranded DNA Viruses in Tomato Cultivars with and without the Ty-1 Gene28 July 2020 | Viruses, Vol. 12, No. 8Ty‐1 , a universal resistance gene against geminiviruses that is compromised by co‐replication of a betasatellite22 November 2019 | Molecular Plant Pathology, Vol. 21, No. 2
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