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Development of a bioelectrode based on catalase enzyme and the novel protic ionic liquid pentaethylenehexammonium acetate (PEHAA)

2019; Elsevier BV; Volume: 280; Linguagem: Inglês

10.1016/j.molliq.2019.02.019

1873-3166

Gala Rosales, Flora Alves, Fábio Costa, Manuel Martín‐Pastor, Valéria Cristina Fernandes, Silvana Mattedi, Jaime Soares Boaventura,

Ionic liquids properties and applications

This work presents the development of a bioelectrode based on catalase enzyme and a novel protic ionic liquid, pentaethylenehexammonium acetate (PEHAA), on a glassy carbon surface. PEHAA was synthesized for the first time and its structure was confirmed by FTIR and NMR. Viscosity and conductivity of PEHAA aqueous mixtures were measured and a Walden plot was used to analyze PEHAA ionicity. The bioelectrode was constructed using layer by layer (LBL) and electrochemical deposition (ECD) techniques. The catalase enzyme was immobilized on the surface of a glassy carbon electrode with the aid of the ionic liquid; the electrodes were evaluated by using cyclic voltammetry at various scan rates. The bioelectrode constructed in this work was stable; it lost only 13% of the initial current after prolonged use. The bioelectrode was electrocatalytically active in the reduction of hydrogen peroxide. On the other hand, the electrodes prepared only by using PEHAA, without catalase, were found to be unresponsive, indicating that the catalytic response is due to catalase and not to PEHAA. However, the presence of the ionic liquid (IL) is fundamental for the immobilization of the enzyme. Consequently, these results indicated that the developed bioelectrodes, based on catalase and PEHAA, are very promising to application as biosensor devices.