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A single-cell molecular map of mouse gastrulation and early organogenesis

2019; Nature Portfolio; Volume: 566; Issue: 7745 Linguagem: Inglês

10.1038/s41586-019-0933-9

1476-4687

Blanca Pijuan-Sala, Jonathan A. Griffiths, Carolina Guibentif, Tom W. Hiscock, Wajid Jawaid, Fernando J. Calero‐Nieto, Carla Mulas, Ximena Ibarra-Soria, Richard C. V. Tyser, Debbie L. L. Ho, Wolf Reik, Shankar Srinivas, Benjamin D. Simons, Jennifer Nichols, John C. Marioni, Berthold Göttgens,

3D Printing in Biomedical Research

Across the animal kingdom, gastrulation represents a key developmental event during which embryonic pluripotent cells diversify into lineage-specific precursors that will generate the adult organism. Here we report the transcriptional profiles of 116,312 single cells from mouse embryos collected at nine sequential time points ranging from 6.5 to 8.5 days post-fertilization. We construct a molecular map of cellular differentiation from pluripotency towards all major embryonic lineages, and explore the complex events involved in the convergence of visceral and primitive streak-derived endoderm. Furthermore, we use single-cell profiling to show that Tal1