Portal de Periódicos da CAPES

Identifying a potential biomarker for primary focal segmental glomerulosclerosis and its association with recurrence after transplantation

2019; Wiley; Volume: 33; Issue: 3 Linguagem: Inglês

10.1111/ctr.13487

1399-0012

Efrat Harel, Jun Shoji, Vivek C. Abraham, Loan Miller, Zoltán Lászik, Tine Thurison, Andrew J. King, Adam B. Olshen, Joey Leung, Gyula Szabó, Byron Hann, Gunilla Høyer‐Hansen, Charles S. Craik, Flavio Vincenti,

Coagulation, Bradykinin, Polyphosphates, and Angioedema

Abstract Background We investigated circulating levels of individual soluble urokinase plasminogen activation receptor (su PAR ) forms to determine if specific circulating fragments of su PAR ( II ‐ III ) and (I) can better serve as clinical biomarkers for focal segmental glomerulosclerosis ( FSGS ) and the risk of recurrence after transplantation. Materials and Methods Serum levels of intact su PAR and its cleaved forms were measured with two assays, ELISA and TR ‐ FIA . Results su PAR levels in healthy controls were significantly lower than those who had glomerular diseases but were not significantly different between FSGS patients and glomerular controls. Intact su PAR (I‐ II ‐ III ) levels were noted to be elevated in glomerular diseases including FSGS . u PAR fragment (I) levels measured with the TR ‐ FIA 4 assay were significantly higher in FSGS (695.4 + 91.29 pMol/L) than glomerular controls (239.1 + 40.45 pMol/L, P = 0.001). However, su PAR (I) levels were not significantly different between recurrent FSGS and nonrecurrent FSGS patients. Conclusion Our analysis of su PAR using the ELISA assay used in all previous studies does not appear to be a useful marker for FSGS nor serve as a predictor for its recurrence after transplantation. The TR ‐ FIA assay results suggest that uPAR (I) is a potential biomarker for FSGS but not of its recurrence.