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Rapid histologic diagnosis using quick fluorescence staining and tissue confocal microscopy

2019; Wiley; Volume: 82; Issue: 6 Linguagem: Inglês

10.1002/jemt.23234

1097-0029

Hongrae Kim, Sunhye Lee, Hyunjin Kim, Hee Jin Chang, Yongdoo Choi, Hong Man Yoon, Myeong‐Cherl Kook, So‐Youn Jung, Youngmi Kwon, Juehyung Kang, Hongki Yoo, Incheon Song, Seungrag Lee, Dae Kyung Sohn,

Nanoplatforms for cancer theranostics

With the development of advanced and minimally invasive surgical techniques, and in view of the functional and cosmetic aspects, the need for rapid and accurate diagnosis during surgery is increasing. This study was conducted to develop a tissue diagnosis method using confocal microscopy after simple tissue staining that does not require freezing and slicing. At present, fluorescence staining with confocal microscopy is not generalized for real-time diagnosis during surgery. In this paper, we propose a fluorescence staining method using Hoechst 33342 and Eosin that does not require tissue freezing and slicing. The proposed method can be used as part of a rapid tissue diagnosis method that is suitable for use in the operating room, although further research is required before it can be applied in clinical practice.