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Lessons from human umbilical cord: gender differences in stem cells from Wharton’s jelly

2019; Elsevier BV; Volume: 234; Linguagem: Inglês

10.1016/j.ejogrb.2018.12.028

1872-7654

Francesca Balzano, Emanuela Bellu, Valentina Basoli, Silvia Dei Giudici, Sara Santaniello, Sara Cruciani, Federica Facchin, Annalisa Oggiano, Giampiero Capobianco, Francesco Dessole, Carlo Ventura, Salvatore Dessole, Margherita Maioli,

Organ and Tissue Transplantation Research

To study the molecular features of mesenchymal stem cells from Wharton Jelly (WJ-MSCs) of umbilical cord to predict their differentiation capacity.Comparison of gene expression from mesenchymal stem cells of male and female umbilical cord SETTING: University hospital PATIENT (S): umbilical cords (n = 12, 6 males and 6 females) retrieved from spontaneous full-term vaginal delivery of healthy women INTERVENTION: we analyzed the expression of the stemness related genes C-MYC, OCT4, SOX2 and NANOG and of the epigenetic modulating gene DNA-methyltransferase 1 (DNMT1).WJ-MSCs were isolated by standard procedures and immunophenotypically characterized. Gene expression analysis of stemness related genes and the epigenetic modulating gene DNMT1 were performed by real-time PCR RESULTS: expression of the OCT4 and DNMT1 genes was significantly higher in WJ- MSCs isolated from male subjects, as compared to MSCs isolated from female-derived WJ. The resulting higher expression of OCT4 and DNMT1 in WJ-MSCs from males as compared with female WJ-MSCs for the first time identifies a specific relationship between stemness genes, an epigenetic modulator, and gender differences.our findings disclose novel biomedical implications in WJ-MSCs related to the sex of the donor, thus providing additional cues to exploit their regenerative potential in allogenic transplantation.