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Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples

2019; Associação Brasileira de Divulgação Científica; Volume: 52; Issue: 2 Linguagem: Inglês

10.1590/1414-431x20188194

1414-431X

Mauren Fernanda Moller dos Santos, F.C.A.C. Oliveira, Renata Kiyomi Kishimoto, Daniela Borri, Fábio Pires de Souza Santos, Paulo Vidal Campregher, Paulo Augusto Achucarro Silveira, Nelson Hamerschlak, Cristóvão Luís Pitangueira Mangueira, Fernando Barroso Duarte, A.H. Crepaldi, Marco Aurélio Salvino, Elvira Deolinda Rodrigues Pereira Velloso,

Chronic Myeloid Leukemia Treatments

Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). The rate of unsuccessful karyotyping was 18.5% and was associated with the use of PB and a low number of nucleated cells (≤7×103/µL) in the sample. High and low cellularity in BM and high and low cellularity in PB samples showed no metaphases in 3.9, 39.7, 41.9, and 84.6% of cases, respectively. Collecting a good BM sample is the key for the success of karyotyping in MN and avoids the use of expensive molecular techniques.