First Report of Grapevine Virus E and Grapevine Virus F in Grapevine in Greece
2019; American Phytopathological Society; Volume: 103; Issue: 6 Linguagem: Inglês
10.1094/pdis-11-18-2108-pdn
ISSN1943-7692
AutoresPolina Panailidou, Leonidas Lotos, Antonio Olmos, Ana Belén Ruiz-García, Félix Morán, C. G. Orfanidou, C.-L. Sassalou, N. I. Katis, Varvara I. Maliogka,
Tópico(s)Plant Disease Resistance and Genetics
ResumoHomePlant DiseaseVol. 103, No. 6First Report of Grapevine Virus E and Grapevine Virus F in Grapevine in Greece PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Grapevine Virus E and Grapevine Virus F in Grapevine in GreeceP. Panailidou, L. Lotos, A. Olmos, A. B. Ruiz-Garcia, F. Moran, C. G. Orfanidou, C.-L. Sassalou, N. I. Katis, and V. I. MaliogkaP. PanailidouLaboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloniki, GreeceSearch for more papers by this author, L. LotosLaboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloniki, GreeceSearch for more papers by this author, A. OlmosInstituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, SpainSearch for more papers by this author, A. B. Ruiz-GarciaInstituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, SpainSearch for more papers by this author, F. MoranInstituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, SpainSearch for more papers by this author, C. G. OrfanidouLaboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloniki, GreeceSearch for more papers by this author, C.-L. SassalouLaboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloniki, GreeceSearch for more papers by this author, N. I. KatisLaboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloniki, GreeceSearch for more papers by this author, and V. I. Maliogka†Corresponding author: V. I. Maliogka; E-mail Address: [email protected]http://orcid.org/0000-0001-5714-2710Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloniki, GreeceSearch for more papers by this authorAffiliationsAuthors and Affiliations P. Panailidou1 L. Lotos1 A. Olmos2 A. B. Ruiz-Garcia2 F. Moran2 C. G. Orfanidou1 C.-L. Sassalou1 N. I. Katis1 V. I. Maliogka1 † 1Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece 2Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain Published Online:19 Apr 2019https://doi.org/10.1094/PDIS-11-18-2108-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Grapevine virus E (GVE) and Grapevine virus F (GVF) are members of the genus Vitivirus (family Betaflexiviridae) (Al Rwahnih et al. 2012; Nakaune et al. 2008) with a worldwide distribution. Even though grapevine viruses A and B, two other vitiviruses, are prevalent in Greek vineyards, no other member of the genus Vitivirus has been reported in Greece so far. In 2017, during a study of the virome of Greek grapevines, one sample (D2.1) from cultivar Dafnia (Institute of Grapevine, Likovrisi, Attiki) was subjected to high-throughput sequencing of total RNA extracted from phloem scrapings using a Plant/Fungi Total RNA Purification Kit (Norgen Biotek Corporation, Canada) on an Illumina NextSeq platform (Lifesequencing, S.L., Spain). The run yielded ∼51 million 150-bp paired-end reads. De novo assembly of these reads and subsequent BLAST (n/x) analysis of the produced contigs revealed sequences of GVE and GVF, among others. Almost complete genomes from both viruses were reconstructed with 71 to 98% and 86 to 89% nucleotide sequence identities to GVE and GVF isolates for which sequences are deposited in databases, respectively. The reconstructed genomes were deposited in GenBank with accession numbers MK490829 for GVE (isolate D2-1/8) and MK490830 and MK490831 for GVF (two different GVF variants coinfecting D2.1, namely D2-1/9, D2-1/13). To confirm the presence of these two viruses in the D2.1 sample, two sets of primers (GVEup [5′-ATGGAGTCAAAAGCGATCMG-3′] and GVEdo [5′-ACCTGTGACTGAGCATCAAATAC-3′]; and GVF_F_4521 [5′-TGTGTGGGCKAARACATA TG-3′] and GVF_R_ 5190 [5′-ATCAGAAAAGATGCTMCTCACCT-3′]) were used to amplify a 574 and a 670-bp fragments from the coat protein and polymerase gene of GVE and GVF, respectively. Sanger sequencing of the amplicons confirmed the presence of both viruses in the analyzed sample. Seventy additional samples from the Institute’s collection were screened for the presence of GVE and GVF, and the viruses were identified in five and 12 vines, respectively. To our knowledge, this is the first report of GVE and GVF in grapevine in Greece.The author(s) declare no conflict of interest.References:Al Rwahnih, M., et al. 2012. J. Virol. 86:9545. https://doi.org/10.1128/JVI.01444-12 Crossref, ISI, Google ScholarNakaune, R., et al. 2008. Arch. Virol. 153:1827. https://doi.org/10.1007/s00705-008-0188-5 Crossref, ISI, Google ScholarThis publication reflects only the authors’ view. The Agency is not responsible for any use that may be made of the information it contains.The author(s) declare no conflict of interest.Funding: This study was partially funded by VirFree. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 734736 (H2020 Marie Skłodowska-Curie Actions: MSCA-RISE-2016-Virfree/734736). COST Action FA1407 (DIVAS) is also acknowledged.DetailsFiguresLiterature CitedRelated Vol. 103, No. 6 June 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionGreen mottle mosaic and leaf deformation symptoms on watermelon (Sui, Li, Shamimuzzaman, Wu, and Ling). Photo credit: K.-S. Ling. Postharvest rot on cucumber caused by Ceratocystis fimbriata (Li, Xu, Zhang, Song, Xie, Sun, and Huang). Photo credit: H. Song. Metrics Article History Issue Date: 6 Jun 2019Published: 19 Apr 2019First Look: 12 Feb 2019Accepted: 7 Feb 2019 Pages: 1440-1440 InformationThis article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 2019.FundingH2020 Marie Skłodowska-Curie ActionsGrant/Award Number: MSCA-RISE-2016-Virfree/734736Keywordspathogen detectionpathogen diversityvitivirusThe author(s) declare no conflict of interest.Cited byVirome of Grapevine Germplasm from the Anapa Ampelographic Collection (Russia)15 June 2022 | Viruses, Vol. 14, No. 6First Report of Grapevine Virus H in Grapevine in GreeceP. Panailidou, L. Lotos, C.-L. Sassalou, E. Gagiano, G. Pietersen, N. I. Katis, and V. I. 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