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Experimental mixture design solvent effects on pigment extraction and antioxidant activity from Coffea arabica L. leaves

2019; Elsevier BV; Volume: 146; Linguagem: Inglês

10.1016/j.microc.2019.01.073

1095-9149

Gustavo Galo Marcheafave, Cláudia Domiciano Tormena, Elis Daiane Pauli, Miroslava Rakočević, Roy E. Bruns, Ieda Spacino Scarmínio,

Heavy Metals in Plants

High-performance liquid chromatography coupled with ultraviolet-visible spectroscopy, using a photodiode array detector (HPLC-PDA) were used to explore different pigments as well as their antioxidant activities in Coffea arabica leaves. Ethanol, dichloromethane and hexane were used for extraction following a simplex-centroid mixture design. Antioxidant capacity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay (DPPH). HPLC-PDA allowed identification of five pigments, pheophytin a, zeaxanthin, crocetin, β-carotene and chlorophyll b. Pheophytin a showed the highest relative abundance followed by zeaxanthin and chlorophyll b with crocetin and β-carotene having even lower amounts. All these pigments exhibited significant antioxidant activities. Ethanol extracts presented the highest relative abundance and antioxidant activities for all these pigments followed by an ethanol: dichloromethane mixture (50:50 v/v). On the other hand, pure hexane had no extraction capacity for zeaxanthin, crocetin, β-carotene and chlorophyll b for the Coffea arabica leaves.