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A Photo‐Crosslinkable Kidney ECM‐Derived Bioink Accelerates Renal Tissue Formation

2019; Wiley; Volume: 8; Issue: 7 Linguagem: Inglês

10.1002/adhm.201800992

2192-2659

Mohamed Ali, Prabha D. Nair, James J. Yoo, Faten Zahran, Anthony Atala, Sang Jin Lee,

Additive Manufacturing and 3D Printing Technologies

Abstract 3D bioprinting strategies in tissue engineering aim to fabricate clinically applicable tissue constructs that can replace the damaged or diseased tissues and organs. One of the main prerequisites in 3D bioprinting is finding an appropriate bioink that provides a tissue‐specific microenvironment supporting the cellular growth and maturation. In this respect, decellularized extracellular matrix (dECM)‐derived hydrogels have been considered as bioinks for the cell‐based bioprinting due to their capability to inherit the intrinsic cues from native ECM. Herein, a photo‐crosslinkable kidney ECM‐derived bioink (KdECMMA) is developed that could provide a kidney‐specific microenvironment for renal tissue bioprinting. Porcine whole kidneys are decellularized through a perfusion method, dissolved in an acid solution, and chemically modified by methacrylation. A KdECMMA‐based bioink is formulated and evaluated for rheological properties and printability for the printing process. The results show that the bioprinted human kidney cells in the KdECMMA bioink are highly viable and mature with time. Moreover, the bioprinted renal constructs exhibit the structural and functional characteristics of the native renal tissue. The potential of the tissue‐specific ECM‐derived bioink is demonstrated for cell‐based bioprinting that could enhance the cellular maturation and eventually tissue formation.