First Report of Onion Yellow Dwarf Virus, Leek Yellow Stripe Virus, and Shallot Latent Virus on Garlic ( Allium sativum ) in Tunisia
2019; American Phytopathological Society; Volume: 103; Issue: 8 Linguagem: Inglês
10.1094/pdis-09-18-1618-pdn
ISSN1943-7692
AutoresChadha Ayed, Armelle Marais, Chantal Faure, Thierry Candresse, Imen Hamdi Nasr, Asma Najar, B. Al-Mohandes Dridi,
Tópico(s)Plant and Fungal Interactions Research
ResumoHomePlant DiseaseVol. 103, No. 8First Report of Onion Yellow Dwarf Virus, Leek Yellow Stripe Virus, and Shallot Latent Virus on Garlic (Allium sativum) in Tunisia PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Onion Yellow Dwarf Virus, Leek Yellow Stripe Virus, and Shallot Latent Virus on Garlic (Allium sativum) in TunisiaC. Ayed, A. Marais, C. Faure, T. Candresse, I. Hamdi, A. Najar, and B. Al-Mohandes DridiC. Ayed†Corresponding author: C. Ayed; E-mail Address: [email protected]http://orcid.org/0000-0003-3041-934XUR13AGR08 Biochimie et Eco-toxicologie environnementale, Institut Supérieur Agronomique de Chott Mariem, Université de Sousse, 4042, Sousse, Tunisia, A. MaraisUMR 1332 Biologie du Fruit et Pathologie, INRA, Université de Bordeaux, CS20032, 33882, Villenave d'Ornon Cedex, France, C. FaureUMR 1332 Biologie du Fruit et Pathologie, INRA, Université de Bordeaux, CS20032, 33882, Villenave d'Ornon Cedex, France, T. Candressehttp://orcid.org/0000-0001-9757-1835UMR 1332 Biologie du Fruit et Pathologie, INRA, Université de Bordeaux, CS20032, 33882, Villenave d'Ornon Cedex, France, I. HamdiLaboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, 1004 ElMenzah, Tunis, Tunisia, A. NajarLaboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, 1004 ElMenzah, Tunis, Tunisia, and B. Al-Mohandes DridiUR13AGR08 Biochimie et Eco-toxicologie environnementale, Institut Supérieur Agronomique de Chott Mariem, Université de Sousse, 4042, Sousse, TunisiaAffiliationsAuthors and Affiliations C. Ayed1 † A. Marais2 C. Faure2 T. Candresse2 I. Hamdi3 A. Najar3 B. Al-Mohandes Dridi1 1UR13AGR08 Biochimie et Eco-toxicologie environnementale, Institut Supérieur Agronomique de Chott Mariem, Université de Sousse, 4042, Sousse, Tunisia 2UMR 1332 Biologie du Fruit et Pathologie, INRA, Université de Bordeaux, CS20032, 33882, Villenave d'Ornon Cedex, France 3Laboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, 1004 ElMenzah, Tunis, Tunisia Published Online:13 Jun 2019https://doi.org/10.1094/PDIS-09-18-1618-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat The potyviruses onion yellow dwarf virus (OYDV) and leek yellow stripe virus (LYSV) are the most important viral pathogens of garlic (Allium sativum L.), whereas carlaviruses and allexiviruses seem to be less detrimental or even latent (Katis et al. 2012). In Tunisia, growers propagate self-produced garlic cloves and/or those from other farmers without phytosanitary control, which facilitates virus transmission. To determine the virus profile of garlic in Tunisia, 33 garlic accessions were collected from major garlic-growing regions and grown in the research farm of the High Agronomic Institute of Chott Mariem. In total, 66 plants (two per accession) were tested for potyviruses and carlaviruses. Ninety percent of leaf samples collected in June 2016 showed virus-like symptoms (yellow stripe, mosaic, and dwarfism). RNA was extracted from each sample (Foissac et al. 2005) and submitted to reverse transcription polymerase chain reaction (RT-PCR) using universal potyvirus-specific primers NIb2F/NIb3R (Zheng et al. 2010). All samples yielded the expected 350-bp product. Sequencing of 18 randomly selected amplicons and BLASTn analysis indicated the presence of OYDV and/or LYSV. Their occurrence was confirmed by RT-PCR using OYDV-specific primers (10214-F, 5′CGTTTGTTTGGCCTGGATGGTAACG3′; 10539-R, 5′GGTCTCYGTAATTCACGC3′), targeting a 325-nucleotide (nt)-long fragment corresponding to the 3′ part of the coat protein (CP) gene, and the 3′ noncoding region for the LYSV-specific primers (LY5P-F, 5′AATCTCAACACAACTTATRC3′; LY2M-R, 5′AGTACGTTGCCTGCTCTGTAG3′), targeting a 720-nt-long fragment of the P1 region of the virus (Yoshida et al. 2012). Direct sequencing of three OYDV and four LYSV amplicons was performed to verify the specificity of the amplifications. The incidences of OYDV and LYSV were estimated at ∼82% (54 of 66 tested samples) and ∼89% (59 of 66), respectively. Approximately 73% (n = 48) of the samples were coinfected with both viruses. Three OYDV isolates (GenBank nos. MH890551 to 53) shared 85 to 90% nt identity with each other and 81 to 92% nt identity with Japanese isolate "Gams" (AB000836). The LYSV isolates (MH890558 to 61) shared 58.1 to 70.42% nt identity in the amplified P1 fragment and between 72 and 80% with the LYSV reference sequence NC004011 (isolate Yuhang GYH from China). Further, isolates from Tunisia shared 46 to 65% of amino acid identity with LYSV reference sequence of P1 protein (NP_734093) and 41 to 60% among them. In parallel, broad-specificity primers (MC2, 5′GCIAARSBIGYICARWSIATYGTITG3′; RMC5, 5′TCICCIGARAAICGCATRATIGC3′) targeting a 358-nt fragment of the RdRp region (Couture et al. unpublished) were used to detect carlaviruses in the same garlic samples. The amplification products from all positive samples (∼41%, 27 of 66 tested plants) were sequenced, revealing in each case the presence of shallot latent virus (SLV). RT-PCRs with specific primers targeting a sequence of 484-nt fragment in the capsid gene (SLV-F, 5′CGTGAAGGCTTYGAGGCCGTTCT3′; SLV-R, 5′AATTCAAACAACGCGCGTA3′) confirmed the presence of this virus in 27 samples. The CP-specific sequences of four specific SLV amplicons showed that they are closely related and shared 88 to 94% nt identity in the amplified fragment. They are most closely related to an Australian garlic isolate (HQ258896), with up to 96.5% nt identity in the CP gene fragment. For the RdRp fragment, these isolates (MH890566 to 69) shared 92 to 96% sequence identity with each other and 92 to 96% with the closest variant, MS/SW/Aus2 (HQ258896). Three of the 66 samples had double infection of SLV and LYSV, and 24 had a mixed infection of all three viruses. This is the first report of OYDV, LYSV, and SLV on garlic in Tunisia. These viruses could be a limiting factor for successful Allium production, and further investigation is necessary to prevent spread of these pathogens. Our future aim is to produce certified virus-free stocks of the most cultivated Tunisian garlic germplasm.The author(s) declare no conflict of interest.References:Foissac, X., et al. 2005. Phytopathology 95:617. https://doi.org/10.1094/PHYTO-95-0617 Link, ISI, Google ScholarKatis, N., et al. 2012. Adv. Virus Res. 84:163. https://doi.org/10.1016/B978-0-12-394314-9.00005-1 Crossref, ISI, Google ScholarYoshida, N., et al. 2012. Arch. Virol. 157:147. https://doi.org/10.1007/s00705-011-1132-7 Crossref, ISI, Google ScholarZheng, L., et al. 2010. Plant Pathol. 59:211. https://doi.org/10.1111/j.1365-3059.2009.02201.x Crossref, ISI, Google ScholarThe author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 103, No. 8 August 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionPistachio fruit infected by Neofusicoccum mediterraneum (Moral et al.). Photo credit: T. J. Michailides. Leaf blight on Hosta ventricosa caused by Pseudomonas syringae pv. syringae (Liu et al.). Photo credit: Z. X. Liu. Metrics Article History Issue Date: 1 Aug 2019Published: 13 Jun 2019First Look: 23 Jan 2019Accepted: 21 Jan 2019 Page: 2143 Information© 2019 The American Phytopathological SocietyThe author(s) declare no conflict of interest.Cited byPhylogenetic and diversity analyses revealed that leek yellow stripe virus population consists of three types: S, L, and N8 November 2022 | Virus Genes, Vol. 59, No. 1First Report of Garlic virus A, Garlic virus B, and Garlic virus C on Garlic (Allium sativum) in TunisiaC. Ayed, I. Hamdi, A. Najar, A. Marais, C. Faure, T. Candresse, and B. Al-Mohandes Dridi14 March 2022 | Plant Disease, Vol. 106, No. 4Leek yellow stripe virusCABI Compendium, Vol. CABI CompendiumShallot latent virusCABI Compendium, Vol. CABI CompendiumOnion yellow dwarf virus (onion yellow dwarf)CABI Compendium, Vol. CABI Compendium
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