First Report of Epicoccum sorghinum Causing Leaf Spot on Paris polyphylla in China
2019; American Phytopathological Society; Volume: 103; Issue: 6 Linguagem: Inglês
10.1094/pdis-11-18-2076-pdn
ISSN1943-7692
AutoresRongtao Fu, C. Chen, Jichun Wang, Daihua Lu, Xun Gong,
Tópico(s)Yeasts and Rust Fungi Studies
ResumoHomePlant DiseaseVol. 103, No. 6First Report of Epicoccum sorghinum Causing Leaf Spot on Paris polyphylla in China PreviousNext DISEASE NOTESFirst Report of Epicoccum sorghinum Causing Leaf Spot on Paris polyphylla in ChinaR. Fu, C. Chen, J. Wang, D. Lu, and X. GongR. Fuhttp://orcid.org/0000-0002-9561-6486Institute of Plant Protection, Sichuan Academy of Agricultural Science, Chengdu 610066, P. R. ChinaKey Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture, Chengdu 610066, Sichuan, China, C. ChenInstitute of Plant Protection, Sichuan Academy of Agricultural Science, Chengdu 610066, P. R. ChinaKey Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture, Chengdu 610066, Sichuan, China, J. WangInstitute of Plant Protection, Sichuan Academy of Agricultural Science, Chengdu 610066, P. R. ChinaKey Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture, Chengdu 610066, Sichuan, China, D. Lu†Corresponding author: D. Lu; E-mail Address: [email protected]Institute of Plant Protection, Sichuan Academy of Agricultural Science, Chengdu 610066, P. R. ChinaKey Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture, Chengdu 610066, Sichuan, China, and X. GongInstitute of Plant Protection, Sichuan Academy of Agricultural Science, Chengdu 610066, P. R. ChinaAffiliationsAuthors and Affiliations R. Fu1 2 C. Chen1 2 J. Wang1 2 D. Lu1 2 † X. Gong1 1Institute of Plant Protection, Sichuan Academy of Agricultural Science, Chengdu 610066, P. R. China 2Key Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture, Chengdu 610066, Sichuan, China Published Online:19 Apr 2019https://doi.org/10.1094/PDIS-11-18-2076-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Paris polyphylla is a perennial medicinal herb of the family Liliaceae that is mostly distributed in southwestern China. The rhizomes of P. polyphylla are a well-known in traditional Chinese medicine and used as antitumor, antimicrobial, and analgesic agents (Zhao et al. 2010). In 2017 and 2018, a severe leaf spot disease was observed on P. polyphylla in Chongzhou city of Sichuan Province. Approximately 35% of plants were infected in the field. Early symptoms appeared as small, water-soaked, chlorotic spots. As the disease progressed, the spots enlarged and developed oval or irregular lesions with necrotic centers and dark brown borders. At the later stage, some of the lesions split through the dried necrotic area, causing extensive leaf senescence. To isolate the causal agent, diseased leaf tissue taken from the margin between necrotic lesions and healthy tissue was surface sterilized with 75% ethanol solution for 45 s, washed three times with sterile ddH2O, plated on potato sucrose agar (PSA), and incubated at 25°C in the dark for 3 days. Mycelia growing from the edges of the tissues were transferred on PSA plates and cultured for 5 days at 25°C. Finally, 20 isolates were obtained. The colonies of these isolates were villose, regular, grayish-green, and approximately 8.0 cm in diameter after 7 days of culture, whereas the reverse side was pale brown. After approximately 21 days of incubation, colonies became gray-brown, secreting scarlet diffusible pigment, and the reverse sides were reddish-brown. Chlamydospores were observed, which were brown to black, with a verrucose surface. Unicellular chlamydospores were 2.3 to 7.8 × 4.8 to 12.4 μm, and multicellular chlamydospores were 4.8 to 22.6 × 13.8 to 56.8 μm. Pycnidia were produced on PSA media and ranged from 90.8 to 210.4 × 120.6 to 240.3 μm. Conidia were ellipsoidal, hyaline, unicellular, and varied from 2.0 to 2.5 × 4.0 to 5.0. The cultural and morphological characteristics corresponded to Epicoccum sorghinum (Zhou et al. 2018). To further confirm this identification, DNA was extracted from three representative isolates and subjected to PCR amplification. The ribosomal internal transcribed spacer (ITS), 28S nrDNA (LSU), actin (ACT), and β-tubulin (TUB) were amplified, purified, and sequenced with the primers ITS1/ITS4, LR7/LROR, ACT-512F/ACT-783R, and T1/βt2b (Aveskamp et al. 2010; O'Donnell et al. 1997; Vilgalys and Hester 1990; White et al. 1990). Sequences from isolate SCCZ16 were deposited in GenBank (accession nos. MK131351 for ITS, MK131352 for LSU, MK131354 for ACT, and MK131355 for TUB). Analysis using the BLAST algorithm of the four genes showed 99 to 100% similarity to the sequences (MH633723, KY000557, MF987526, and MH141600) of E. sorghinum. For pathogenicity tests, leaves from 3-year-old healthy plants of P. polyphylla were inoculated with 5-mm mycelial plugs (6 days old) of three isolates growing on PSA, and control plants were inoculated with sterile PSA plugs. There were four plants for each treatment. All plants were individually covered with plastic bags for 48 h to maintain high humidity and incubated in a glasshouse at 25 ± 2°C. All experiments were carried out twice. The typically water-soaked necrotic lesions were observed 5 days after inoculation. No disease symptoms were observed on control leaves. E. sorghinum was reisolated from the inoculated leaves, thus confirming Koch's postulates. To our knowledge, this is the first report of E. sorghinum causing leaf spot on P. polyphylla in China.The author(s) declare no conflict of interest.References:Aveskamp, M. M., et al. 2010. Stud. Mycol. 65:1. https://doi.org/10.3114/sim.2010.65.01 Google ScholarO'Donnell, K., et al. 1997. Mol. Phylogenet. Evol. 7:103. https://doi.org/10.1006/mpev.1996.0376 Crossref, ISI, Google ScholarVilgalys, R., and Hester, M. 1990. J. Bacteriol. 172:4238. https://doi.org/10.1128/jb.172.8.4238-4246.1990 Crossref, ISI, Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. Crossref, Google ScholarZhao, J. L., et al. 2010. Molecules 15:7961. https://doi.org/10.3390/molecules15117961 Crossref, ISI, Google ScholarZhou, H., et al. 2018. Plant Dis. 102:1039. https://doi.org/10.1094/PDIS-11-17-1757-PDN Link, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: The work was financially supported by Sichuan Science and Technology Program (2018JY0111) and the Financial Innovation and Promotion Project of Sichuan Province (2016GYSH-014).DetailsFiguresLiterature CitedRelated Vol. 103, No. 6 June 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionGreen mottle mosaic and leaf deformation symptoms on watermelon (Sui, Li, Shamimuzzaman, Wu, and Ling). Photo credit: K.-S. Ling. Postharvest rot on cucumber caused by Ceratocystis fimbriata (Li, Xu, Zhang, Song, Xie, Sun, and Huang). Photo credit: H. Song. Metrics Article History Issue Date: 6 Jun 2019Published: 19 Apr 2019First Look: 15 Feb 2019Accepted: 11 Feb 2019 Page: 1426 Information© 2019 The American Phytopathological SocietyFundingSichuan Science and Technology ProgramGrant/Award Number: 2018JY0111Financial Innovation and Promotion Project of Sichuan ProvinceGrant/Award Number: 2016GYSH-014KeywordsEpicoccum sorghinumITSLSUACTTUBThe author(s) declare no conflict of interest.Cited byEpicoccum sorghinum as leaf spot disease-causing pathogen in ginger (Zingiber officinale Rosc.) in ChinaCrop Protection, Vol. 170Epicoccum spp. Causing Maize Leaf Spot in Heilongjiang Province, ChinaXi Xu, Jingjing Li, Xilang Yang, Li Zhang, Shuo Wang, Guijin Shen, Bing Hui, Jialei Xiao, Changjian Zhou, Xiangjing Wang, Junwei Zhao, and Wensheng Xiang21 November 2022 | Plant Disease, Vol. 106, No. 12Sequence Data, Functional Annotation, and Relationship Analysis Between mRNAs and Long Noncoding RNAs from Tea Leaves During Infection by the Fungal Pathogen Epicoccum sorghinumChen Huang, Hongke Huang, Zhongqiu Xia, Yuqin Yang, Xinyue Jiang, Yuanyou Yang, Delu Wang, Xiangyang Li, and Zhuo Chen15 September 2022 | Molecular Plant-Microbe Interactions, Vol. 35, No. 9Epicoccum species: ubiquitous plant pathogens and effective biological control agents22 January 2021 | European Journal of Plant Pathology, Vol. 159, No. 4First Report of Epicoccum sorghinum Causing Leaf Spot on Atractylodes lancea in Hubei Province, ChinaYongxi Du, Yang Xu, Jingyi Jiang, Xiufu Wan, Chuanzhi Kang, Tielin Wang, and Lanping Guo4 August 2020 | Plant Disease, Vol. 104, No. 11
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