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BIBTEX RIS

Extracellular vesicles derived from cancer‐associated fibroblasts induce the migration and invasion of oral squamous cell carcinoma

2019; Taylor & Francis; Volume: 8; Issue: 1 Linguagem: Inglês

10.1080/20013078.2019.1578525

2001-3078

Maurício Rocha Dourado, Johanna Korvala, Pirjo Åström, Carine Ervolino de Oliveira, Nilva K. Cervigne, Luciana Souto Mofatto, Débora Campanella Bastos, Ana Camila Messetti, Edgard Graner, Adriana Franco Paes Leme, Ricardo D. Coletta, Tuula Salo,

Cancer-related molecular mechanisms research

As one of the most abundant constituents of the tumour microenvironment (TME), cancer-associated fibroblasts (CAF) display critical roles during tumour progression and metastasis. Multiple classes of molecules including growth factors, cytokines, proteases and extracellular matrix proteins, are produced by CAF to act as mediators of the stroma-tumour interactions. One of the main channels for this communication is associated with extracellular vesicles (EV), which are secreted particles loaded with protein and genetic information. In this study, we evaluated the effects of EV derived from CAF primary human cell lines (n = 5) on proliferation, survival, migration, and invasion of oral squamous cell carcinoma (OSCC) cells. As controls, EV from human primary-established normal oral fibroblasts (NOF, n = 5) were used. Our in vitro assays showed that CAF-EV significantly induces migration and invasion of OSCC cells and promote a disseminated pattern of HSC-3 cell invasion in the 3D organotypic assay. Furthermore, gene expression analysis of EV-treated cancer cells revealed changes in the pathways associated with tumour metabolism and up-regulation of tumour invasion genes. Our findings suggest a significant role of CAF-EV in promoting the migration and invasion of OSCC cells, which are related to the activation of cancer-related pathways.