CRISPResso2 provides accurate and rapid genome editing sequence analysis
2019; Nature Portfolio; Volume: 37; Issue: 3 Linguagem: Inglês
10.1038/s41587-019-0032-3
ISSN1546-1696
AutoresKendell Clement, Holly A. Rees, Matthew C. Canver, Jason M. Gehrke, Rick Farouni, Jonathan Y. Hsu, Mitchel A. Cole, David R. Liu, J. Keith Joung, Daniel E. Bauer, Luca Pinello,
Tópico(s)RNA and protein synthesis mechanisms
ResumoGenome editing technologies are rapidly evolving, and analysis of deep sequencing data from target or off-target regions is necessary for measuring editing efficiency and evaluating safety. However, no software exists to analyze base editors, perform allele-specific quantification or that incorporates biologically-informed and scalable alignment approaches. Here, we present CRISPResso2 to fill this gap and illustrate its functionality by experimentally measuring and analyzing the editing properties of six genome editing agents.
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