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Hachimoji DNA and RNA: A genetic system with eight building blocks

2019; American Association for the Advancement of Science; Volume: 363; Issue: 6429 Linguagem: Inglês

10.1126/science.aat0971

1095-9203

Shuichi Hoshika, Nicole A. Leal, Myong‐Jung Kim, Myong‐Sang Kim, Nilesh B. Karalkar, Hyo‐Joong Kim, Alison M. Bates, Norman E. Watkins, Holly A. SantaLucia, Adam J. Meyer, Saurja DasGupta, Joseph A. Piccirilli, Andrew D. Ellington, John SantaLucia, Millie M. Georgiadis, Steven A. Benner,

Advanced biosensing and bioanalysis techniques

Expanding the genetic code DNA and RNA are naturally composed of four nucleotide bases that form hydrogen bonds in order to pair. Hoshika et al. added an additional four synthetic nucleotides to produce an eight-letter genetic code and generate so-called hachimoji DNA. Coupled with an engineered T7 RNA polymerase, this expanded DNA alphabet could be transcribed into RNA. Thus, new forms of DNA that add information density to genetic biopolymers can be generated that may be useful for future synthetic biological applications. Science , this issue p. 884