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BIBTEX RIS

Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)

2019; Brazilian Society of Genetics; Volume: 42; Issue: 1 Linguagem: Inglês

10.1590/1678-4685-gmb-2017-0293

ISSN

1678-4685

Autores

Juliana Rodrigues Bombonato, Isabel A. S. Bonatelli, Gislaine Angélica Rodrigues Silva, Evandro M. Moraes, Daniela C. Zappi, Nigel P. Taylor, Fernando Faria Franco,

Tópico(s)

Plant and animal studies

Resumo

The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Ariocarpus, Echinocactus, Polaskia and Pilosocereus, in four taxa of the genus Cereus: C. fernambucensis subsp. fernambucensis, C. fernambucensis subsp. sericifer, C. jamacaru and C. insularis. Nine microsatellite loci were amplified in Cereus resulting in 35.2% of success in transferability, which is higher than the average rate of 10% reported in the literature for cross-genera transferability in eudicots. The genetic variation in the transferred markers was sufficient to perform standard clustering analysis, indicating each population as a cohesive genetic cluster. Overall, the amount of genetic variation found indicates that the transferred SSR markers might be useful in large-scale population studies within the genus Cereus.

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