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BIBTEX RIS

An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts

2017; Elsevier BV; Volume: 21; Linguagem: Inglês

10.1016/j.scr.2017.03.011

ISSN

1876-7753

Autores

Enrique Sosa, Rachel Kim, Ernesto J. Rojas, Linzi Hosohama, Jon D. Hennebold, Kyle E. Orwig, Amander T. Clark,

Tópico(s)

Virus-based gene therapy research

Resumo

The rhesus macaque induced pluripotent stem cell (riPSC) line, UCLAi090-A (riPSC90), was generated from rhesus embryonic fibroblast (REF) cells called REF90. REF90 cells and the riPSC90 line were authenticated by short tandem repeat analysis and had a normal male (42, XY) karyotype. The riPSC90 line expressed markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4, and generated teratomas after transplantation into immunocompromised mice. riPSC90 could be used in parallel with riPSC89, which was derived from REFs cultured from a different rhesus macaque embryo (Sosa et al. 2016).

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