Produção Nacional
Revisado por pares
Recovery and purification of 503 antigen from Leishmania i. chagasi with simultaneous removal of lipopolysaccharides: Influence of immobilized metals and elution strategies during expanded bed adsorption (EBA)
2018; Taylor & Francis; Volume: 41; Issue: 19-20 Linguagem: Inglês
10.1080/10826076.2019.1565829
ISSN1520-572X
AutoresAna Laura Oliveira de Sá Leitão, Maria Cecília Bezerra Caldas, Carlos Eduardo de Araújo Padilha, Cleitiane Nogueira da Costa, Patrícia Maria Rocha, Francisco Canindé de Sousa Júnior, Gorete Ribeiro de Macêdo, Everaldo Silvino dos Santos,
Tópico(s)Acute Lymphoblastic Leukemia research
ResumoThe present study aimed to evaluate different metals immobilized onto the Streamline™ chelating resin for purification of 503 antigen from Leishmania i. chagasi expressed in E. coli M15 by expanded bed adsorption (EBA), as well as to investigate the removal of the lipopolysaccharides (LPS) during the EBA washing step. Batch adsorption tests were performed using five metal ions (Cu+2, Ni+2, Zn+2, Co+2, and Fe+3) at concentrations of 0.1, 0.5, and 0.8 M coupled to the Streamline™ chelating resin. Three concentrations of the Triton X-114 surfactant (0.01%, 0.05%, and 0.1%) were evaluated during the EBA washing step in order to estimate the minimum amount of surfactant required to remove LPS, aiming to obtain the 503 antigen without this contaminant. Results showed that Cu+2 was the metal with the highest adsorption capacity of 503 antigen, under the three concentrations studied in this work. The addition of low concentrations of the nonionic surfactant Triton X-114 to the ALE washing step promoted the removal of LPS (>99.0%) that were initially present in the samples for all concentrations investigated. Additionally, a 15.0% recovery of the 503 antigen and a purification factor of approximately 3.0 was obtained directly from the unclarified homogenate.
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