Produção Nacional
Revisado por pares
First Report of Cotton Leafroll Dwarf Virus Infecting Cotton in Georgia, U.S.A.
2019; American Phytopathological Society; Volume: 103; Issue: 7 Linguagem: Inglês
10.1094/pdis-12-18-2197-pdn
ISSN1943-7692
AutoresAfsha Tabassum, Sudeep Bag, P. Roberts, N. D. Suassuna, Peng W. Chee, Jared R. Whitaker, Kassie Conner, Judith K. Brown, R. L. Nichols, Robert C. Kemerait,
Tópico(s)Insect-Plant Interactions and Control
ResumoHomePlant DiseaseVol. 103, No. 7First Report of Cotton Leafroll Dwarf Virus Infecting Cotton in Georgia, U.S.A. Previous DISEASE NOTESFirst Report of Cotton Leafroll Dwarf Virus Infecting Cotton in Georgia, U.S.A.A. Tabassum, S. Bag, P. Roberts, N. Suassuna, P. Chee, J. R. Whitaker, K. N. Conner, J. Brown, R. L. Nichols, and R. C. KemeraitA. TabassumDepartment of Plant Pathology, The University of Georgia, Tifton, GA 31793, U.S.A., S. Bag†Corresponding author: S. Bag; E-mail Address: [email protected]http://orcid.org/0000-0003-3724-7341Department of Plant Pathology, The University of Georgia, Tifton, GA 31793, U.S.A., P. RobertsDepartment of Entomology, The University of Georgia, Tifton, GA 31793, U.S.A., N. SuassunaEmbrapa Algodão, 75375-000 Santo Antônio de Goiás, GO, Brazil, P. CheeDepartment of Crops and Soil Science, The University of Georgia, Tifton, GA 31793, U.S.A., J. R. WhitakerDepartment of Crops and Soil Science, The University of Georgia, Tifton, GA 31793, U.S.A., K. N. ConnerAlabama Cooperative Extension System, Auburn University, Auburn, AL 36849, U.S.A., J. BrownSchool of Plant Science, University of Arizona Tucson, AZ 85721, U.S.A., R. L. NicholsCotton Inc., Cary, NC 27513, U.S.A., and R. C. KemeraitDepartment of Plant Pathology, The University of Georgia, Tifton, GA 31793, U.S.A.AffiliationsAuthors and Affiliations A. Tabassum1 S. Bag1 † P. Roberts2 N. Suassuna3 P. Chee4 J. R. Whitaker4 K. N. Conner5 J. Brown6 R. L. Nichols7 R. C. Kemerait1 1Department of Plant Pathology, The University of Georgia, Tifton, GA 31793, U.S.A. 2Department of Entomology, The University of Georgia, Tifton, GA 31793, U.S.A. 3Embrapa Algodão, 75375-000 Santo Antônio de Goiás, GO, Brazil 4Department of Crops and Soil Science, The University of Georgia, Tifton, GA 31793, U.S.A. 5Alabama Cooperative Extension System, Auburn University, Auburn, AL 36849, U.S.A. 6School of Plant Science, University of Arizona Tucson, AZ 85721, U.S.A. 7Cotton Inc., Cary, NC 27513, U.S.A. Published Online:2 May 2019https://doi.org/10.1094/PDIS-12-18-2197-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat During the 2018 growing season, cotton plants in several fields in Georgia were observed with symptoms that included leaf curling, reddening and drooping of leaves, subsequent distortion of leaf growth above the nodes where reddened leaves were first observed, and shortening of upper internodes and their discoloration to deep green. These symptoms were visible in the upper portion of the plants, resembling "cotton blue" disease caused by cotton leafroll dwarf virus (CLRDV) (family Luteoviridae, genus Polerovirus). The symptoms were observed sporadically with less than 10% disease incidence within affected fields. CLRDV is a phloem-limited virus with single-stranded, positive-sense RNA genome, transmitted by aphids (Aphis gossypii) in a persistent, circulative, nonpropagative manner (Distéfano et al. 2010; Sharman et al. 2015). A total of 93 symptomatic and asymptomatic leaves and petioles were collected from several different commercial cotton fields in the southern part of the state. Total RNA was extracted using the modified cetyltrimethylammonium bromide method (Sharman et al. 2015). Complementary DNA (cDNA) was synthesized from 2.5 µg of total RNA using Superscript III reverse transcription (Invitrogen, U.S.A.) and specific reverse primers targeting different open reading frames (ORFs) of the virus genome. The cDNA was used for polymerase chain reaction (PCR) with primers CLRDV3675F and Pol3982R targeting ORF 3 of the virus (310-bp product, Sharman et al. 2015) and primers 17 and 18 targeting ORF 5 (1,065-bp product, Distéfano et al. 2010). A new set of primers, SB11F (5′-AGGTTTTCTGGTAGCAGTACCAATATCAACGTTA-3′) and SB11R (5′-TATCTTGCATTGTGGATTTCCCTCATAA-3′), was developed to amplify the 803-bp fragment spanning complete ORF 3 and ORF 4, encoding virus coat protein and movement protein genes. Using the three different sets of primers, products of predicted sizes were amplified from the symptomatic tissues, but not from asymptomatic tissues collected from fields and negative controls including asymptomatic leaves from plants grown in an insect-free controlled growth chamber and DNase/RNase free water (Invitrogen). Reverse transcription PCR products were gel purified, cloned into TOPO-TA cloning vector (Invitrogen), and sequenced using Sanger sequencing (GeneScript, NJ). The consensus sequence of three clones for ORF 3 and 4 from this study (MK290759 and MK290760) when analyzed using NCBI-BLASTn showed 97% sequence identity with the Brazilian and Argentinean isolates (KF906261.1 and KF359947.1, respectively) and many other isolates from South American and Asian countries. ORF 3 and 4 sequences from Georgia shared 98 to 99% identity among themselves. The partial sequences for ORF 5 (MK513938 and MK513939) shared 95% identity with the isolate from Argentina (KF359946.1 and KF359947.1). The presence of the virus was detected in all the symptomatic samples collected from 14 counties in Georgia, covering the entire cotton growing region of the state. To the best of our knowledge, this is the first report of CLRDV in Georgia, U.S.A. CLRDV infection of cotton has been reported in other cotton-growing regions in Africa, Asia, South America, and, more recently, in Alabama, U.S.A. (Avelar et al. 2018; Corrêa et al. 2005; Distéfano et al. 2010; Mukherjee et al. 2012). With its rapid spread, this virus could pose an imminent threat and cause severe economic losses to the cotton industry. Further research is needed to elucidate epidemiology, symptomatology, vector transmission, and crop losses owing to CLRDV in the United States.The author(s) declare no conflict of interest.References:Avelar, S., et al. 2018. Plant Dis. 103:592. Link, ISI, Google ScholarCorrêa, R., et al. 2005. Arch. Virol. 150:1357. Crossref, ISI, Google ScholarDistéfano, A. J., et al. 2010. Arch. Virol. 155:1849. Crossref, ISI, Google ScholarMukherjee, A. K., et al. 2012. New Dis. Rep. 25:22. Crossref, Google ScholarSharman, M., et al. 2015. Australas. Plant Dis. Notes 10:24. Crossref, ISI, Google ScholarA. Tabassum and S. Bag contributed equally.The author(s) declare no conflict of interest.Funding: The authors acknowledge the funding and support provided by Hatch Act and State of Georgia funding, Georgia Cotton Commission (project number 18-101GCC), and Cotton Incorporated (project number 18-780).DetailsFiguresLiterature CitedRelated Vol. 103, No. 7 July 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionApple cultivar Joya Cripps Red lesions caused by Colletotrichum fructicola (Nodet et al.). Photo credit: P. Nodet. Symptoms of Lotus powdery mildew caused by Erysiphe takamatsui (Zhou et al.). Photo credit: C. Liang. Symptoms of tar spot (Phyllachora maydis) on maize leaves (Dalla Lana et al.). Photo credit: F. Dalla Lana. Metrics Article History Issue Date: 20 Jun 2019Published: 2 May 2019First Look: 4 Mar 2019Accepted: 24 Feb 2019 Page: 1803 Information© 2019 The American Phytopathological SocietyFundingHatch Act and State of Georgia fundingGeorgia Cotton CommissionGrant/Award Number: project number 18-101GCCCotton IncorporatedGrant/Award Number: project number 18-780Keywordscotton blue diseasecotton leafroll dwarf virusatypicalGeorgiacottonThe author(s) declare no conflict of interest.Cited byDifferential sensitivities of photosynthetic component processes govern oxidative stress levels and net assimilation rates in virus-infected cotton20 July 2023 | Photosynthesis Research, Vol. 158, No. 1Analysis of Cotton Leafroll Dwarf Virus P0 Gene Sequences from South Carolina Reveals Low Variability Among IsolatesWilliam W. 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