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Automated Colony Counter for Single Plate Serial Dilution Spotting

2019; Springer Science+Business Media; Linguagem: Inglês

10.1007/978-3-030-13469-3_48

1611-3349

Dimitria T. Boukouvalas, Peterson Adriano Belan, Cintia Raquel Lima Leal, Renato Araújo Prates, Sidnei Alves de Araújo,

Genomics and Phylogenetic Studies

This paper discusses the automated visual identification and quantification of colony forming units (CFU) in Single Plate Serial Dilution Spotting (SP-SDS) through correlation-based granulometry under uncontrolled lighting conditions. There are many different approaches in the literature based on images captured under controlled conditions, which is not the real life situation of laboratories that present high variation in illuminating conditions resulting in low contrast between bacterial colonies and background, background noise, and in addition, high variation in CFU features. Furthermore, SP-SDS has been widely used due to its reduction in the use of resources, but most of previous approaches are not capable of counting separately the number of CFU present in each dilution zone. In that sense, our study focuses on analyzing real images taken at laboratory day-to-day conditions and proposes an approach suitable for real laboratory practice with high accuracies.