Artigo Produção Nacional Revisado por pares

Mangifera indica leaves extract and mangiferin modulate CB1 and PPARγ receptors and others markers associated with obesity

2019; Elsevier BV; Volume: 56; Linguagem: Inglês

10.1016/j.jff.2019.03.003

ISSN

2214-9414

Autores

Larissa Froede Brito, Douglas Costa Gontijo, Renata Celi Lopes Toledo, Rafael Mazioli Barcelos, Alaı́de Braga de Oliveira, Geraldo Célio Brandão, Lirlândia P. Sousa, Sônia Machado Rocha Ribeiro, João Paulo Viana Leite, Luciano Gomes Fietto, José Humberto de Queiróz,

Tópico(s)

Adipokines, Inflammation, and Metabolic Diseases

Resumo

This study aimed phytochemical characterization (UPLC-DAD-MS/MS) of ethanolic extract of the leaves from Mangifera indica (EMI) and Mangiferin (MAN), analysis of the cytotoxic (MTT) and anti-inflammatory potential (expression of TNF-α) of EMI and MAN in vitro. In addition, was evaluate the effect on the mRNA expression of genes (CB1, PPARγ, adiponectin, resistin and leptin) associated with adipogenesis in adipose tissue of rats fed a cafeteria diet. Thus, wistar rats were treated by gavage with EMI and MAN for several days (according to the post and co-treatments). The adipose tissue was weighed and checked the expression of different markers by RT-PCR. The presence of MAN as major compound in EMI was verified. Both EMI and MAN were not cytotoxic, with lower EMI expression of TNF-α. Furthermore, EMI and MAN had proadipogenic action on post-treatment, while in the co-treatment, EMI attenuated the effect of adipogenesis and MAN increased the adipogenic process.

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