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Biological evaluation and molecular docking study of metabolites from Salvadora Persica L. Growing in Egypt

2019; SAGE Publishing; Volume: 15; Issue: 61 Linguagem: Inglês

10.4103/pm.pm_361_18

0976-4062

Ahmed M. Metwaly, Mohammed M. Ghoneim, WaelM Afifi, Mohamed Ibrahim, Mohamed Elagawany, M Hasanzadeh Khayat, Mohamed Abou-Taleb,

Phytochemistry and Biological Activities

Pharmacognosy Magazine ,2019,15,61,232-237.DOI:10.4103/pm.pm_361_18Published:March 2019Type:Original Article Authors:Mohammed M Ghoneim, Wael M Afifi, Mohamed Ibrahim, Mohamed Elagawany, Maan T Khayat, Mohamed H Aboutaleb, and Ahmed M Metwaly Author(s) affiliations:Mohammed M Ghoneim1, Wael M Afifi2, Mohamed Ibrahim3, Mohamed Elagawany4, Maan T Khayat5, Mohamed H Aboutaleb6, Ahmed M Metwaly2 1Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt; Department of Natural Products, School of Pharmacy, Almaarefa Colleges for Science and Technology, Riyadh, Saudia Arabia 2Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt 3Department of Organic Chemistry, College of Pharmacy, Misr University for Science and Technology, Al-Motamayez District, Damnhour, Egypt 4Department of Pharmaceutical Organic Chemistry, College of Pharmacy, Damnhour University, Damnhour, Egypt 5Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudia Arabia 6Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Horus University, New Damietta 34517, Egypt Abstract:Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a resistant staph bacterium to several antibiotics causing several lives-threating diseases such as pneumonia and sepsis. Meswak, Salvadora persica, exhibited promising antimicrobial properties before. Objective: Exploring the anti-MRSA activity of S. persica L. metabolites and its mechanism of action on a molecular level. Materials and Methods: Structure elucidation of the isolated metabolites was carried out by spectroscopic data (one-dimensional and two-dimensional nuclear magnetic resonance). The biological activities of the isolated metabolites against MRSA were evaluated and the molecular mode of action against the dehydrosqualene synthase enzyme have been done. Results: Four compounds have been isolated and identifies to be; apigenin (1), luteolin (2), astragalin (3), and kaempferol-3-O-rhamnoside (4). Compounds 1–4 showed good anti-MRSA activities with IC50 values of 10.3, 11.5, 3.5, and 4.5 μg/mL, respectively. In consistent, astragalin and kaempferol-3 rhamnoside showed close high docking scores. Herein, we are reporting the molecular determinates of activity of these new scaffolds as anti-MRSA, which would be of great importance to developing new anti-MRSA candidates. Keywords:Anti-methicillin-resistant Staphylococcus aureus, antimicrobial activities, dehydrosqualene synthase enzyme, docking molecular study, Salvadora persicaView:PDF (1.91 MB)