First Report of Leaf Blight of Rice Caused by Pantoea ananatis and Pantoea dispersa in Malaysia
2019; American Phytopathological Society; Volume: 103; Issue: 7 Linguagem: Inglês
10.1094/pdis-12-18-2299-pdn
ISSN1943-7692
AutoresWai Keat Toh, P. C. Loh, Hann Ling Wong,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoHomePlant DiseaseVol. 103, No. 7First Report of Leaf Blight of Rice Caused by Pantoea ananatis and Pantoea dispersa in Malaysia PreviousNext DISEASE NOTESFirst Report of Leaf Blight of Rice Caused by Pantoea ananatis and Pantoea dispersa in MalaysiaW. K. Toh, P. C. Loh, and H. L. WongW. K. TohFaculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia, P. C. LohFaculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia, and H. L. Wong†Corresponding author: H. L. Wong; E-mail Address: [email protected]http://orcid.org/0000-0001-8903-1812Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, MalaysiaAffiliationsAuthors and Affiliations W. K. Toh P. C. Loh H. L. Wong † Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia Published Online:16 May 2019https://doi.org/10.1094/PDIS-12-18-2299-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat In September to December 2016, the most severe leaf blight disease outbreak in the last 30 years occurred in the paddy field of Sekinchan, Selangor, Malaysia, causing 50 to 70% loss of the crop yield. During this outbreak, the newly introduced rice cultivar MR 284 suffered the most damage. Infected rice leaf blades showed brownish stripes, which subsequently turned pale and dry. Symptomatic leaves from the MR 284 plants were cut into 1-cm2 pieces, surface sterilized, and plated on peptone sucrose agar (Poulin et al. 2014). Yellowish bacterial colonies that were obtained after incubation at 28°C for 2 days were purified by streaking on nutrient agar. Identification of six randomly chosen bacterial isolates was performed using the BioMérieux Vitek 2 microbial identification system, which classified the isolates as Pantoea spp. with 93 to 96% identity probabilities. Subsequently, 16S rRNA gene amplification was performed using the universal primers 27F and 1492R. BLASTn analysis of the partial 16S rRNA gene sequences from the six isolates revealed that the sequences (1,421 bp) from two isolates, designated as PA strains, were identical to those of Pantoea ananatis (GenBank accession no. CP028033.1), and the sequences (1,435 bp) of four isolates, designated as PC strains, were identical to those of Pantoea dispersa (GenBank accession no. AB273743.1). The partial 16S rRNA gene sequences of PA and PC strains were deposited at GenBank under accession numbers MK578186 and MK263020, respectively. Pathogenicity assays of PA and PC strains were conducted on 60-day-old rice plants of cultivar MR 284 using the clipping method (Kauffman et al. 1973). The bacterial strains were cultured overnight on nutrient broth, and the inocula were adjusted to 108 cells/ml. Three fully extended leaves from the second and third tillers of each plant were inoculated by cutting at 3 to 4 cm from the leaf tip. Control plants were mock inoculated with sterile saline solution. The experiment was performed in duplicate. From 3 to 14 days after bacterial inoculation, the inoculated leaves showed progressive necrosis with color change from pale green, brown, and pale, whereas the control plants remained symptomless. Bacterial colonies were reisolated from inoculated leaves, producing colonies that were morphologically identical to those of PA and PC strains. The identities of these bacterial isolates were further verified to be the inocula PA and PC strains by 16S rRNA gene sequencing, thus fulfilling the Koch's postulates. Previous reports had established Pantoea spp. as rice pathogens (Kini et al. 2017; Lee et al. 2010; Mondal et al. 2011). To our knowledge, this is the first report of rice leaf blight caused by P. ananatis and P. dispersa in Malaysia. The isolation of these pathogens allows newly developed cultivars to be more adequately tested before these cultivars are introduced to the farmers for mass cultivation.The author(s) declare no conflict of interest.References:Kauffman, H. E., et al. 1973. Plant Dis. Rep. 57:537. ISI, Google ScholarKini, K., et al. 2017. Plant Dis. 101:242. https://doi.org/10.1094/PDIS-06-16-0940-PDN Link, ISI, Google ScholarLee, H. B., et al. 2010. Plant Dis. 94:1372. https://doi.org/10.1094/PDIS-05-10-0374 Link, ISI, Google ScholarMondal, K. K., et al. 2011. Plant Dis. 95:1582. https://doi.org/10.1094/PDIS-06-11-0533 Link, ISI, Google ScholarPoulin, L., et al. 2014. Plant Dis. 98:1423. https://doi.org/10.1094/PDIS-02-14-0132-PDN Link, ISI, Google ScholarThe author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 103, No. 7 July 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionApple cultivar Joya Cripps Red lesions caused by Colletotrichum fructicola (Nodet et al.). Photo credit: P. Nodet. Symptoms of Lotus powdery mildew caused by Erysiphe takamatsui (Zhou et al.). Photo credit: C. Liang. Symptoms of tar spot (Phyllachora maydis) on maize leaves (Dalla Lana et al.). Photo credit: F. Dalla Lana. Metrics Article History Issue Date: 20 Jun 2019Published: 16 May 2019First Look: 18 Mar 2019Accepted: 13 Mar 2019 Pages: 1764-1764 Information© 2019 The American Phytopathological SocietyKeywordsPantoealeaf blightriceThe author(s) declare no conflict of interest.Cited byDevelopment of specific PCR primer sets for detecting Pantoea dispersa, a potential biocontrol agent against rice seed-borne diseases caused by Burkholderia pathogens19 October 2023 | Journal of General Plant Pathology, Vol. 90, No. 1Pantoea Bacteria Isolated from Three Thrips ( Frankliniella occidentalis , Frankliniella intonsa , and Thrips tabaci ) in Korea and Their Symbiotic Roles in Host Insect Development13 March 2023 | Journal of Microbiology and Biotechnology, Vol. 33, No. 6Rice and paddy industry in Malaysia: governance and policies, research trends, technology adoption and resilience14 June 2023 | Frontiers in Sustainable Food Systems, Vol. 7Diverse Virulence Attributes of Pantoea alfalfae sp. nov. 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