Produção Nacional
Revisado por pares
Agrobacterium-Mediated Transformation of Diaporthe schini Endophytes Associated with Vitis labrusca L. and Its Antagonistic Activity Against Grapevine Phytopathogens
2019; Springer Science+Business Media; Volume: 59; Issue: 2 Linguagem: Inglês
10.1007/s12088-019-00787-0
ISSN0973-7715
AutoresAretusa Cristina Felber, Júlio César Polonio, Ravely Casarotti Orlandelli, Alessandra Tenório Costa, Eliane Papa Ambrosio‐Albuquerque, Renata Assis Castro, Maria Carolina Quecine-Verdi, João Lúcio de Azevedo, João Alencar Pamphile, Káthia Socorro Mathias Mourão,
Tópico(s)Microbial Natural Products and Biosynthesis
ResumoFungus-caused diseases are among the greatest losses in grapevine culture. Biological control of pathogens by endophytes may be used to decrease fungicide application rates and environmental impacts. Previously, Diaporthe sp. B46-64 and C27-07 were highlighted as antagonists of grapevine phytopathogens. Herein, molecular multigene (ITS-TUB-TEF1) identification and phylogenetic analysis allowed the identification of these endophytes as belonging to Diaporthe schini species. Agrobacterium tumefaciens-mediated transformation was employed for obtaining 14 stable and traceable gfp- or DsRed-expressing transformants, with high transformation efficiency: 96% for the pFAT-GFP plasmid and 98% for pCAM-DsRed plasmid. Transformants were resistant to hygromycin B with gene hph confirmed by polymerase chain reaction and proved to be mitotically stable, expressing the fluorescent phenotype, with morphological differences in the colonies when compared with wild strains. In vitro antagonism tests revealed an increased antagonistic activity of some transformant strains. The current genetic transformation of D. schini mediated by A. tumefaciens proved to be an efficient technique within the randomized insertion of reporter genes for the monitoring of the strain in the environment.
Referência(s)