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Chemically cross-linked polyacrylonitrile. A DMSO compatible NMR alignment medium for measurement of residual dipolar couplings and residual chemical shift anisotropies

2019; Elsevier BV; Volume: 302; Linguagem: Inglês

10.1016/j.jmr.2019.03.005

1096-0856

Daiane S. Carvalho, Danilo G. B. da Silva, Fernando Hallwass, Armando Navarro‐Vázquez,

Protein Structure and Dynamics

Chemically cross-linked polyacrylontrile polymer gels, have been prepared as an alignment medium compatible with DMSO-d6. These gels allow measurement of residual dipolar couplings (RDCs) and residual chemical shift anisotropies (RCSAs) with good accuracy as tested with brucine and α-santonin natural compounds. The gels can be reversibly compressed allowing easy measurement of RCSAs. They also present good physical homogeneity, clean HSQC spectra with little background 1H signals, and allow unambiguous referencing of 13C spectra for RCSA extraction.