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BIBTEX RIS

Process development for the production of prebiotic fructo-oligosaccharides by penicillium citreonigrum

2019; Elsevier BV; Volume: 282; Linguagem: Inglês

10.1016/j.biortech.2019.03.053

1873-2976

Clarisse Nobre, Ana Karoline Caitano do Nascimento, Soraia P. Silva, Elisabete Coelho, Manuel A. Coimbra, Maria Taciana Holanda Cavalcanti, J. A. Teixeira, Ana Lúcia Figueiredo Porto,

Enzyme Production and Characterization

A new isolated P. citreonigrum URM 4459 was selected to produce fructooligosaccharides (FOS) in an efficient, economical and fast one-step fermentation. Optimal culture conditions were stablished by experimental design. Experiments run in bioreactor resulted in a high yield, content, productivity and purity of FOS (0.65 ± 0.06 gFOS/ginitial Sucrose, 126.3 ± 0.1 g/L, 2.28 ± 0.08 g/L.h and 61 ± 0%). The FOS mixture was purified up to 92% (w/w) with an activated charcoal column. FOS produced were able to promote lactobacilli and bifidobacteria growth. Higher bacteria cell density was obtained for microbial-FOS mixtures than commercial Raftilose® P95. Some strains grew even faster in the FOS mixture produced than in all other carbon sources. FOS were resistant to the simulated gastrointestinal conditions. A high amount of a reducing trisaccharide was identified in the FOS produced mixture, possibly neokestose, which may explain the great prebiotic potential of the FOS.