Produção Nacional
Revisado por pares
Detection of Colletotrichum boninense Causing Leaf Anthracnose on Alcantarea imperialis in Brazil
2019; American Phytopathological Society; Volume: 103; Issue: 8 Linguagem: Inglês
10.1094/pdis-02-19-0410-pdn
ISSN1943-7692
AutoresP. R. Meneses, Keilor da Rosa Dorneles, Cristiano Bellé, Victoria Moreira, Viviana Gaviria-Hernández, C. R. J. de Farias,
Tópico(s)Plant pathogens and resistance mechanisms
ResumoHomePlant DiseaseVol. 103, No. 8Detection of Colletotrichum boninense Causing Leaf Anthracnose on Alcantarea imperialis in Brazil PreviousNext DISEASE NOTESDetection of Colletotrichum boninense Causing Leaf Anthracnose on Alcantarea imperialis in BrazilP. R. Meneses, K. R. Dorneles, C. Bellé, V. L. Moreira-Nuñez, V. Gaviria-Hernández, and C. R. J. de FariasP. R. Meneses†Corresponding author: P. R. Meneses; E-mail Address: [email protected]http://orcid.org/0000-0002-0758-8850Departamento de Fitossanidade, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, 96010900, Capão do Leão, RS, Brazil, K. R. Dorneleshttp://orcid.org/0000-0001-8098-1539Departamento de Fitossanidade, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, 96010900, Capão do Leão, RS, Brazil, C. BelléDepartamento de Fitossanidade, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, 96010900, Capão do Leão, RS, Brazil, V. L. Moreira-NuñezDepartamento de Fitossanidade, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, 96010900, Capão do Leão, RS, Brazil, V. Gaviria-HernándezDepartamento de Fitossanidade, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, 96010900, Capão do Leão, RS, Brazil, and C. R. J. de FariasDepartamento de Fitossanidade, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, 96010900, Capão do Leão, RS, BrazilAffiliationsAuthors and Affiliations P. R. Meneses † K. R. Dorneles C. Bellé V. L. Moreira-Nuñez V. Gaviria-Hernández C. R. J. de Farias Departamento de Fitossanidade, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, 96010900, Capão do Leão, RS, Brazil Published Online:11 Jun 2019https://doi.org/10.1094/PDIS-02-19-0410-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Bromeliads (Alcantarea imperialis Carrière) is an extremely important flowering plant in Brazil and commonly grown and commercialized as an ornamental plant (Kurita and Tamaki 2014). In September 2017, plants with severe spots on the leaves were found in a commercial field in Pelotas, Rio Grande do Sul state, Brazil. The symptoms comprised large circular to elliptical leaf spots ranging from 10 to 40 mm in diameter. Individual spots were sunken and light brown in color, with some appearing black because of the sporulation of fungus on the leaf surface. Leaf tissues (5 × 5 mm) from the lesion margins were surface sterilized (with 1% sodium hypochlorite for 10 min, followed by rinsing with sterile distilled water) and plated on potato dextrose agar at 25°C with a 12-h photoperiod. The cultures were light gray, with cottony aerial mycelium that became darker with age and color ranging from whitish to dark gray on the reverse side of the colonies. The fungus initially produced gray mycelium, followed by orange conidial masses. The conidia were hyaline, smooth-walled, aseptate, straight, cylindrical to clavate, apex round, and measuring 13.7 (± 1.8) × 5.5 (± 0.5) μm, with a length-to-width ratio of 2.5. Molecular characterization was performed by amplifying and sequencing the internal transcribed spacer (ITS), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), chitin synthase 1 (CHS-1), actin (ACT), and calmodulin (CAL) gene regions, using the method and primers described by Damm et al. (2012). Sequences of the studied DNA regions were submitted to GenBank (ITS, MK286012; GAPDH, MK286456; CHS-1, MK286457; ACT, MK286458; and CAL, MK286459). BLAST searches revealed 99 to 100% identity with the existing sequences, including ex-holotype CBS 123755, of Colletotrichum boninense (ITS, JQ005153; GAPDH, JQ005240; CHS-1, JQ005327; ACT, JQ005501; TUB2, JQ005588; and CAL, JQ005674). To verify the pathogenicity of the fungus, a controlled infection was performed in a greenhouse. Six 4-month-old whole A. imperialis seedlings from container nurseries were inoculated by spraying on the adaxial surface of the leaves with a spore suspension (106 conidia/ml) of C. boninense, and six plants, with control treatments, were sprayed with sterile water. All plants were covered with plastic bags. After 2 days of inoculation, the bags were removed. Tests were performed in a greenhouse at 25°C under natural daylight conditions. After 12 days of inoculation, the inoculated plants exhibited typical symptoms similar to those previously observed in the field, whereas the control plants remained symptom-free. The experiment was performed twice. C. boninense isolate was successfully reisolated from the inoculated leaves and confirmed by gene sequence analysis. Thus, the causal agent causing leaf spots on A. imperialis was identified as C. boninense. This is the first report on the infection of C. boninense on A. imperialis worldwide (Farr and Rossman 2018). This finding has great importance for Brazilian flower growers because effective management measures are essential for disease control once the fungus is well-established in a determined geographic area.The author(s) declare no conflict of interest.References:Damm, U., et al. 2012. Stud. Mycol. 73:1. Crossref, ISI, Google ScholarFarr, D. F., and Rossman, A. Y. 2018. Fungal Databases, Syst. Mycol. Microbiol. Lab., ARS, USDA. Retrieved 12 January 2018 from https://nt.ars-grin.gov/fungaldatabases/. Google ScholarKurita, F. M. K., and Tamaki, V. 2014. Acta Scientiarum 36:279. Google ScholarThe author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 103, No. 8 August 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionPistachio fruit infected by Neofusicoccum mediterraneum (Moral et al.). Photo credit: T. J. Michailides. Leaf blight on Hosta ventricosa caused by Pseudomonas syringae pv. syringae (Liu et al.). Photo credit: Z. X. Liu. Metrics Article History Issue Date: 1 Aug 2019Published: 11 Jun 2019First Look: 19 Mar 2019Accepted: 22 Feb 2019 Page: 2125 Information© 2019 The American Phytopathological SocietyKeywordsfungiornamentalspathogen detectionThe author(s) declare no conflict of interest.Cited byFirst Report of Anthracnose Caused by Colletotrichum boninense on Spotted Laurel in ChinaHuanhuan Liu, Kexin Zhu, Chaozhi Li, Chunhua Wei, Fang Li, Fenggang Luan, Dong Li, and Qingni Song8 February 2023 | Plant Disease, Vol. 107, No. 2Morphology, Phylogeny and Pathogenicity of Colletotrichum menglaense sp. nov., Isolated from Air in China26 September 2021 | Pathogens, Vol. 10, No. 10Colletotrichum species and complexes: geographic distribution, host range and conservation status29 September 2021 | Fungal Diversity, Vol. 110, No. 1Characterization and Pathogenicity of Colletotrichum Species on Philodendron tatei cv. Congo in Gansu Province, ChinaLonghai Xue, Yongwen Zhang, Tingyu Duan, Mengyuan Li, James F. White, Yong Liu, and Chunjie Li20 August 2020 | Plant Disease, Vol. 104, No. 10
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