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Toxoplasma gondii causes increased ICAM-1 and serotonin expression in the jejunum of rats 12 h after infection

2019; Elsevier BV; Volume: 114; Linguagem: Inglês

10.1016/j.biopha.2019.108797

1950-6007

Maria José Pastre, Lucas Casagrande, Marcelo Biondaro Goís, Letícia Sarturi Pereira-Severi, Carlos Alberto Miqueloto, João Luís Garcia, Gessilda de Alcântara Nogueira de Melo, Débora de Mello Gonçales Sant’Ana,

Herpesvirus Infections and Treatments

To analyze the remodeling dynamics of total collagen, type I and III, the expression of ICAM-1 and 5-HT in the jejunum of rats.Twenty-eight Wistar rats were randomly assigned to two experimental groups: the control group (CG, n = 7) and the infected group (receiving 5,000 sporulated T. gondii oocysts - ME49 strain, genotype II, n = 21). Seven infected rats each at 6 (G6), 12 (G12), and 24 (G24) hours post infection were sacrificed and segments of jejunum were collected for standard histological, histochemical, and immunohistochemistry processing techniques.The infection promoted ICAM-1 and 5-HT expression, type III collagen, and total mast cell increases. However, it also caused a reduction in the area occupied by type I collagen fibers, and in submucosa thickness, and caused ganglion and peri-ganglion alterations.The structural damage caused by toxoplasmic infection is intense during the first 24 h post inoculation. At peak dissemination, from 12 to 24 h, there is an increase in ICAM-1 and 5-HT expression, with intense migration of mast cells to the site of infection. There was also a reduction in submucosa thickness, and an effective loss of extracellular matrix (ECM) organization, which included changes in the dynamics of type I and III total collagen deposition.