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Xanthene Dyes and Green LED for the Inactivation of Foodborne Pathogens in Planktonic and Biofilm States

2019; Wiley; Volume: 95; Issue: 5 Linguagem: Inglês

10.1111/php.13104

1751-1097

Alex Fiori da Silva, Adriele Rodrigues dos Santos, Daliah Alves Coelho Trevisan, Edinéia Bonin, Camila Fabiano de Freitas, Andréia Farias Pereira Batista, Noboru Hioka, Manuel Simões, Jane Martha Graton Mikcha,

Photodynamic Therapy Research Studies

Abstract This study evaluated the rose bengal‐ and erythrosine‐mediated photoinactivation against Salmonella Typhimurium and Staphylococcus aureus planktonic and sessile cells using green LED as a light source. The free‐living or 2‐day‐old biofilm cells were treated with different concentrations of the photosensitizing agents and subjected to irradiation. Only 5 min photosensitization with rose bengal at 25 nmol L −1 and 75 μ mol L −1 completely eliminated S. aureus and S . Typhimurium planktonic cells, respectively. Erythrosine at 500 nmol L −1 and 5 min of light exposure also reduced S. aureus planktonic cells to undetectable levels. Eradication of S. aureus biofilms was achieved when 500 μ mol L −1 of erythrosine or 250 μ mol L −1 of rose bengal was combined with 30 min of irradiation. Scanning electron microscopy allowed the observation of morphological changes in planktonic cells and disruption of the biofilm architecture after photodynamic treatment. The overall data demonstrate that rose bengal and erythrosine activated by green LED may be a targeted strategy for controlling foodborne pathogens in both planktonic and sessile states.