First Report that Colletotrichum aenigma Causes Leaf Spots on Camellia japonica in China
2019; American Phytopathological Society; Volume: 103; Issue: 8 Linguagem: Inglês
10.1094/pdis-01-19-0224-pdn
ISSN1943-7692
AutoresShuhua Yang, Hanting Wang, Yan-Jun Yi, Lingling Tan,
Tópico(s)Mycorrhizal Fungi and Plant Interactions
ResumoHomePlant DiseaseVol. 103, No. 8First Report that Colletotrichum aenigma Causes Leaf Spots on Camellia japonica in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report that Colletotrichum aenigma Causes Leaf Spots on Camellia japonica in ChinaS. Yang, H. X. Wang, Y. J. Yi, and L. L. TanS. YangShandong Province Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China, H. X. WangDepartment of Science and Technology, Qingdao Agricultural University, Qingdao 266109, China, Y. J. YiShandong Province Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China, and L. L. Tan†Corresponding author: L. L. Tan; E-mail Address: tanlingling80@163.comhttp://orcid.org/0000-0003-4560-6746Shandong Province Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, ChinaAffiliationsAuthors and Affiliations S. Yang1 H. X. Wang2 Y. J. Yi1 L. L. Tan1 † 1Shandong Province Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China 2Department of Science and Technology, Qingdao Agricultural University, Qingdao 266109, China Published Online:4 Jun 2019https://doi.org/10.1094/PDIS-01-19-0224-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Camellia japonica L., also known as common camellia or rose of winter, is a member of the family Theaceae. C. japonica is mainly cultivated in Asian countries such as South Korean, Japan, and China. In particular, it is the floral emblem of Qingdao in China. In March 2018, about 40% of common camellia leaves were observed to have leaf spots in a city park of Qingdao, China. These diseased leaves had circular or irregularly shaped spots with gray color in the center and dark brown on the edge of the lesions. Black particles were scattered around the leaf spots. Small tissue pieces from the edge of the leaf spots were surface sterilized in 70% alcohol for 1 min, rinsed in sterile distilled water once, and then placed on potato dextrose agar (PDA). One fungus was consistently isolated, and the resulting colonies were cottony with white to gray color. After the incubation on PDA for 2 weeks, dark brown conidiomata with setae were observed. Brown setae had one to two septa (30.3 to 80.5 × 4.3 to 5.6 μm). Conidia were single celled (13.0 to 18.3 × 5.0 to 6.3 μm), cylindrical, and straight. Based on the colony and conidial morphology, the isolated fungus was identified as Colletotrichum gloeosporioides species complex (Phoulivong et al. 2010; Weir et al. 2012). Sequences of the internal transcribed spacer region of rDNA (GenBank accession no. MK414786), β-tubulin gene (MK424810), calmodulin gene (MK424811), actin gene (MK424812), chitin synthase gene (MK424813), and glyceraldehyde-3-phosphate dehydrogenase gene (MK424814) were amplified and analyzed from one single isolate, CA-CJ-01 (Weir et al. 2012). These sequences had 99 to 100% nucleotide identity to those genes of Colletotrichum aenigma (MF380961.1, MG717317.1, MG717318.1, MG717314.1, MG717316.1, and MH327271.1, respectively) in GenBank. The pathogenicity test of CA-CJ-01 was performed on 10 healthy, prewounded leaves with 15 µl of conidial suspension (106 conidia/ml) placed on the left side of leaves, with 15 µl of distilled water placed on the right side of leaves as a control treatment. The small branches of C. japonica were then covered with plastic bags to maintain 100% relative humidity at 25°C for 8 days. The experiment was repeated twice. After 8 days, CA-CJ-01 resulted in leaf spots similar to those previously observed on infected leaves in the field, whereas the control leaves were asymptomatic. The reisolated fungi from the symptomatic tissues were identical to CA-CJ-01 in colonial and conidial features, fulfilling Koch's postulates. C. aenigma could cause anthracnose in other Camellia spp. in China, such as Camellia sasanqua (Chen et al. 2019) and Camellia sinensis (Wang et al. 2016). In addition, C. camelliae-japonicae was reported to cause anthracnose on C. japonica (Hou et al. 2016). The putative C. aenigma is a weak pathogen, and to our knowledge, this is the first report of C. aenigma causing leaf spots on C. japonica in the world, providing an important basis for the disease management of C. japonica leaf spots in the future.The author(s) declare no conflict of interest.References:Chen, X., et al. 2019. Plant Dis. doi.org/10.1094/PDIS-11-18-1971-PDN Google ScholarHou, L. W., et al. 2016. Mycosphere. 7:1111. https://doi.org/10.5943/mycosphere/si/2c/4 Crossref, ISI, Google ScholarPhoulivong, S., et al. 2010. Fungal Divers. 44:33. https://doi.org/10.1007/s13225-010-0046-0 Crossref, ISI, Google ScholarWang, Y. C., et al. 2016. Sci. Rep. 6:35287. https://doi.org/10.1038/srep35287 Crossref, ISI, Google ScholarWeir, B. S., et al. 2012. Mycology 73:115. Google ScholarS. Yang and H. X. Wang contributed equally.The author(s) declare no conflict of interest.Funding: This work was supported by the National Natural Science Foundation of China (21776149 and 31872650), the Shandong Provincial Key Research and Development Plan (2016GSF121010), and Cooperation Project Program of Qingdao Medscience & SPKLAM (6602418027).DetailsFiguresLiterature CitedRelated Vol. 103, No. 8 August 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionPistachio fruit infected by Neofusicoccum mediterraneum (Moral et al.). Photo credit: T. J. Michailides. 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