First Report of Pantoea ananatis Causing Leaf Blight Disease of Rice in Peninsular Malaysia
2019; American Phytopathological Society; Volume: 103; Issue: 8 Linguagem: Inglês
10.1094/pdis-01-19-0191-pdn
ISSN1943-7692
AutoresMohammad Malek Faizal Azizi, Dzarifah Zulperi, M. A. Rahman, B. Abdul-Basir, Norashiken Othman, Siti Izera Ismail, Erneeza Mohd Hata, Ina Salwany Md Yasin, M. A. F. Abdullah,
Tópico(s)Plant Pathogens and Fungal Diseases
ResumoHomePlant DiseaseVol. 103, No. 8First Report of Pantoea ananatis Causing Leaf Blight Disease of Rice in Peninsular Malaysia PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Pantoea ananatis Causing Leaf Blight Disease of Rice in Peninsular MalaysiaM. M. F. Azizi, D. Zulperi, M. A. A. Rahman, B. Abdul-Basir, N. A. Othman, S. I. Ismail, E. M. Hata, M. Y. Ina-Salwany, and M. A. F. AbdullahM. M. F. AziziDepartment of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia, D. Zulperi†Corresponding author: D. Zulperi; E-mail Address: [email protected]http://orcid.org/0000-0002-3406-9730Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia, M. A. A. RahmanDepartment of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia, B. Abdul-BasirDepartment of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia, N. A. OthmanDepartment of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia, S. I. IsmailDepartment of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia, E. M. HataDepartment of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia, M. Y. Ina-SalwanyDepartment of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia, and M. A. F. AbdullahAgribolics Technology Sdn Bhd, UPM-MTDC Technology Centre, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaAffiliationsAuthors and Affiliations M. M. F. Azizi1 D. Zulperi1 † M. A. A. Rahman1 B. Abdul-Basir1 N. A. Othman1 S. I. Ismail1 E. M. Hata1 M. Y. Ina-Salwany2 M. A. F. Abdullah3 1Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia 2Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia 3Agribolics Technology Sdn Bhd, UPM-MTDC Technology Centre, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia Published Online:6 Jun 2019https://doi.org/10.1094/PDIS-01-19-0191-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat The genus Pantoea contains pathogenic bacteria causing destructive diseases on rice (Oryzae sativa L.) because they reduce the quality, hence affecting the yield of rice production. Rice infected by Pantoea species can exhibit severe diseases such as leaf blight, stem necrosis, grain discoloration, and damaged germplasm of rice seeds (Mondal et al. 2011). In September 2017 and April 2018, bacterial leaf blight disease of rice (MR269 and CL varieties) was observed in rice fields located in the states of Selangor and Kedah, Malaysia, with 80% disease incidence. Disease symptoms observed were water-soaked stripes with yellowing color, which later turned into brown stripes on the upper part of leaves (Kini et al. 2017). The causal agent was isolated from symptomatic leaf pieces after surface sterilization using 75% ethanol for 30 s and rinsing thrice in sterilized water containing 1% NaClO. Samples were macerated in sterilized water, streaked on semiselective King's B agar medium, and incubated for 48 h at 28°C. Ten representative isolates (PA1, PA3, PA5, PA6, PA7, PA8, PA9, PA10, PA11, and PA12) were isolated from the diseased samples. Bacterial colonies were yellow pigmented, raised, irregular border, and translucent with smooth margin, resembling the characteristics of Pantoea ananatis (Mondal et al. 2011). All isolates were subjected to biochemical tests, revealing gram-negative facultative anaerobe, motile, positive for catalase and gelatin tests, hydrolyzing starch, not producing hydrosulfuric acid, indole positive, and capable of producing acetoin. The DNA of all isolates was extracted using a Geneaid DNA Isolation Kit (Geneaid Biotech, Taiwan). Polymerase chain reaction (PCR) amplification based on the gyrb gene fragment developed from 26 genomes of Pantoea species was performed on all isolates, each producing a ∼600-bp amplicon (Kini et al. 2017). The amplicons were sequenced, and the sequences were submitted to GenBank database (accession nos. MH698458, MH698460, MH698462, MH698463, MH698464, MH698465, MH698466, MH698467, MH698468, and MH698469). A BLASTn search revealed 100% nucleotide identity of all isolates to P. ananatis ARC60 reference strain (accession no. KX385187). The phylogenetic tree constructed from all isolates based on the gyrb gene sequences indicated 99% similarity to P. ananatis reference strains (accession nos. KX385187, KX342014, and KF554589). PCR amplification with P. ananatis-specific gene, PANA_1080, which encodes for a hypothetical protein of the pathogen, produced a ∼900-bp amplicon each (Asselin et al. 2016), and a BLASTn search showed 96% identity to the P. ananatis LMG 20103 reference strain (accession no. CP001875). The nucleotide sequences of all isolates were later deposited to GenBank database (accession nos. MK348547 to MK348556). To test pathogenicity, 10 ml of 108 CFU/ml bacterial suspension of each isolate was inoculated into 35-day-old rice seedlings of MR269 and CL varieties by using a leaf-clipping method, and they were kept in the greenhouse with temperature ranging from 26 to 35°C, performed in triplicate (Ke et al. 2017). Control rice seedlings were inoculated with sterilized water. All isolates of P. ananatis produced symptoms within 2 weeks postinoculation. Symptoms appeared similar to those of natural infections, including yellowish necrotic water-soaked lesion. Control rice seedlings remained asymptomatic. Bacteria were reisolated from symptomatic leaves and further identified as P. ananatis by PANA_1080 gene sequencing, fulfilling the Koch's postulates. To the best of our knowledge, this is the first report of P. ananatis causing leaf blight disease of rice in Malaysia. With the aid of molecular-based approaches, a better understanding on the taxonomy of P. ananatis will help to improve and develop effective disease control strategies against this wide-spreading bacterial pathogen of rice in Malaysia.The author(s) declare no conflict of interest.References:Asselin, J. A. E., et al. 2016. Plant Dis. 100:836. https://doi.org/10.1094/PDIS-08-15-0941-RE Link, ISI, Google ScholarKe, Y., et al. 2017. Bio Protoc. 7:e2568. Crossref, ISI, Google ScholarKini, K., et al. 2017. Plant Dis. 101:241. https://doi.org/10.1094/PDIS-06-16-0939-PDN Link, ISI, Google ScholarMondal, K. K., et al. 2011. Plant Dis. 95:1582. https://doi.org/10.1094/PDIS-06-11-0533 Link, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: Funding was provided by Universiti Putra Malaysia (grant no. IPM 9494600).DetailsFiguresLiterature CitedRelated Vol. 103, No. 8 August 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionPistachio fruit infected by Neofusicoccum mediterraneum (Moral et al.). Photo credit: T. J. Michailides. Leaf blight on Hosta ventricosa caused by Pseudomonas syringae pv. syringae (Liu et al.). Photo credit: Z. X. Liu. Metrics Article History Issue Date: 1 Aug 2019Published: 6 Jun 2019First Look: 15 Apr 2019Accepted: 10 Apr 2019 Page: 2122 Information© 2019 The American Phytopathological SocietyFundingUniversiti Putra MalaysiaGrant/Award Number: IPM 9494600Keywordsprokaryotesfield cropscereals and grainspathogen detectionThe author(s) declare no conflict of interest.Cited byLeaf blight disease of rice caused by Pantoea: profile of an increasingly damaging disease in rice16 November 2022 | Tropical Plant Pathology, Vol. 48, No. 1Beneficial Effect and Potential Risk of Pantoea on Rice Production4 October 2022 | Plants, Vol. 11, No. 19Advanced diagnostic approaches developed for the global menace of rice diseases: a review5 April 2022 | Canadian Journal of Plant Pathology, Vol. 44, No. 5Current and emerging molecular technologies for the diagnosis of plant diseases – An overview30 April 2022 | Journal of Experimental Biology and Agricultural Sciences, Vol. 10, No. 2Pantoea ananatis (fruitlet rot of pineapple)CABI Compendium, Vol. CABI CompendiumAssociations of Pantoea with Rice Plants: As Friends or Foes?16 December 2021 | Agriculture, Vol. 11, No. 12Clarification of the etiology of peach bacteria leaf shot hole caused by Pantoea spp. in Henan Province, China, based on morphological characteristics and multi-locus sequences analysis17 July 2021 | European Journal of Plant Pathology, Vol. 161, No. 2Isolation and Characterization of Novel Phages Targeting Xanthomonas oryzae: Culprit of Bacterial Leaf Blight Disease in RicePHAGE, Vol. 2, No. 3An Efficient Inoculation Technique to Assess the Pathogenicity of Pantoea Species Associated to Bacterial Blight of RiceBIO-PROTOCOL, Vol. 10, No. 17First report of Pantoea ananatis causing fruitlet blight of Nagpur mandarin ( Citrus reticulata ) in India17 January 2020 | New Disease Reports, Vol. 41, No. 1
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