High‐quality draft genome sequence of Gaiella occulta isolated from a 150 meter deep mineral water borehole and comparison with the genome sequences of other deep‐branching lineages of the phylum Actinobacteria
2019; Wiley; Volume: 8; Issue: 9 Linguagem: Inglês
10.1002/mbo3.840
ISSN2045-8827
AutoresRita Severino, Hugo J.C. Froufe, Cristina Barroso, Luciana Albuquerque, Alexandre Lobo‐da‐Cunha, Milton S. da Costa, Conceição Egas,
Tópico(s)Infective Endocarditis Diagnosis and Management
ResumoAbstract Gaiella occulta strain F2‐233 T (=CECT 7815 = LMG 26412), isolated from a 150 meter deep mineral water aquifer, was deemed a candidate for high‐quality draft genome sequencing because of the rare environment from which it was isolated. The draft genome sequence (QQZY00000000) of strain F2‐233 T is composed of approximately 3 Mb, predicted 3,119 protein‐coding genes of which 2,545 were assigned putative functions. Genome analysis was done by comparison with the other deep‐branching Actinobacteria neighbors Rubrobacter radiotolerans , Solirubrobacter soli and Thermoleophilum album . The genes for the tricarboxylic acid cycle, gluconeogenesis and pentose phosphate pathway, were identified in G. occulta , R. radiotolerans , S. soli and T. album genomes. Genes of the Embden–Meyerhof–Parnas pathway and nitrate reduction were identified in G. occulta , R. radiotolerans and S. soli , but not in the T. album genome. Alkane degradation is precluded by genome analysis in G. occulta . Genes involved in myo ‐inositol metabolism were found in both S. soli and G. occulta genomes. A Calvin–Benson–Bassham (CBB) cycle with a type I RuBisCO was identified in G. occulta genome, as well. However, experimental growth under several conditions was negative and CO 2 fixation could not be proven in G. occulta .
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