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Coconut Water as an Extender Component for Cooled Equine Sperm

2019; Elsevier BV; Volume: 78; Linguagem: Inglês

10.1016/j.jevs.2019.03.213

1542-7412

Lucas Santiago Brasileiro, Lorenzo Segabinazzi, Erika Bezerra de Menezes, Cristiane Clemente de Mello Salgueiro, Guilherme Novello, Verônica Flores da Cunha Scheeren, Marco Antônio Alvarenga, José Ferreira Nunes,

Sesame and Sesamin Research

The aim of this study was to evaluate the effect of coconut water as a component of extender in different formulations for cooling equine sperm. One ejaculate of fourteen stallions was collected. Sperm was diluted to 50 × 106 sperm/mL using five different extenders: ACP-105: powdered coconut water extender (ACP-105, ACP Biotecnologia, Brazil); ACP-Milk: ACP-105 + 20 g/L of skimmed milk; ACP-EY 2.5%: ACP-105 + 2.5% of egg yolk; ACP-EY 5%: ACP-105 + 5% of egg yolk; and BotuSêmen (Botupharma, Botucatu, Brazil) and cooled in passive cooling device (BotuFlex, Botupharma, Botucatu, Brazil) at 5 and 15°C for 24 hours. Sperm kinetics and plasma membrane integrity (PMI) were evaluated by computer-assisted sperm analysis and fluorescence staining, respectively, at T0 (before cooling) and T24 (24 hours after cooling). Sperm kinetics did not differ at T0 among groups (P > .05); however, at T24, these parameters were significantly lower in ACP-105 (5°C, total motility [TM]: 9.2 ± 3.6%; progressive motility [PM]: 2.7 ± 1.6%; percentage of fast-moving spermatozoa [RAP]: 4.8 ± 3.0%; 15°C, TM: 10.6 ± 3.0%; PM: 1.1 ± 0.5%; RAP: 4.8 ± 1.9%) and ACP-EY 5% (5°C, TM: 28.0 ± 6.3%; PM: 5.7 ± 1.8%; RAP: 15.9 ± 6.0%; 15°C, TM: 30.0 ± 6.0%; PM: 6.9 ± 2.1%; RAP: 17.6 ± 5.3%) compared with BotuSêmen (5°C, TM: 66.2 ± 5.6%; PM: 21.1 ± 2.8%; RAP: 53.9 ± 6.1%; 15°C, TM: 63.4 ± 5.4%; PM: 17.2 ± 2.8%; RAP: 51.4 ± 6.3%) (P < .05). All groups exhibited similar PMI at tested moments and cooling temperatures (5°C: 83%; 15°C: 84%) (P > .05). Further studies are necessary to evaluate powdered coconut water in different compositions of sperm extender; however, coconut-based extender as used in this study was not an alternative to preserve sperm parameters of cooled equine sperm.