Portal de Periódicos da CAPES

Integrated Printed Microfluidic Biosensors

2019; Elsevier BV; Volume: 37; Issue: 10 Linguagem: Inglês

10.1016/j.tibtech.2019.03.009

0167-9430

Jacky Loo, Aaron Ho Pui Ho, Anthony Turner, Wing Cheung Mak,

Microfluidic and Capillary Electrophoresis Applications

The fast turnaround time of POC microfluidic biosensors has become increasingly important in resource-limited locations for sample-to-answer diagnostics. Printing techniques used to fabricate 2D, 3D, or 4D microfluidics yield both simple and complex microfluidic configurations to suit particular applications. Fabrication of printed microfluidics is enhanced by high printing speeds and resolution, and advanced printing approaches and materials. Combinational printing techniques and materials, such as 3D printing and responsive polymers, are facilitating the development of new printed microfluidic biosensors. The major goal in developing integrated printed microfluidic biosensors is to combine printed microfluidics with printed recognition elements and printed transducers. Integrated printed microfluidic biosensors are one of the most recent point-of-care (POC) sensor developments. Fast turnaround time for production and ease of customization, enabled by the integration of recognition elements and transducers, are key for on-site biosensing for both healthcare and industry and for speeding up translation to real-life applications. Here, we provide an overview of recent progress in printed microfluidics, from the 2D to the 4D level, accompanied by novel sensing element integration. We also explore the latest trends in integrated printed microfluidics for healthcare, especially POC diagnostics, and food safety applications. Integrated printed microfluidic biosensors are one of the most recent point-of-care (POC) sensor developments. Fast turnaround time for production and ease of customization, enabled by the integration of recognition elements and transducers, are key for on-site biosensing for both healthcare and industry and for speeding up translation to real-life applications. Here, we provide an overview of recent progress in printed microfluidics, from the 2D to the 4D level, accompanied by novel sensing element integration. We also explore the latest trends in integrated printed microfluidics for healthcare, especially POC diagnostics, and food safety applications. self-contained integrated analytical device that combines a biological recognition element with a transducer used for detection of an analyte in a quantitative or semiquantitative manner. current gold-standard method relying on enzyme-linked antibodies for detecting protein markers. prokaryotic immune system using the Cas9 enzyme to recognize and specifically cleave the DNA strand complementary to the CRISPR sequence, a family of DNA sequences in the genomes of prokaryotes. methodology to streamline production when needed without prestorage. It aims to reduce times within the production cycle, including the time, space, and labor for delivery of stock from inventory. miniaturized device that combines various laboratory functions on a paper substrate. lowest concentration of the target that is distinguished from a blank with a stated confidence level. actuation of fluid or droplet with a volume below microliter, typically from picoliter to microliter, in a microenvironment, such as microchannels, in a controlled manner. nontoxic, optically clear, silicon-based organic polymeric compound with hydrophobic properties, commonly used in the fabrication of microfluidics and medical devices. on-site diagnostic test performed next to, or by, the patient with minimal assistance. comprise nucleotides or peptides for specific interaction with target analyte. automated performance with minimal or no user interaction from the time the raw sample is inserted until the result is produced (the answer). process the signals from recognition elements and give out measurable data as output.