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Butyrate Protects Mice from Clostridium difficile-Induced Colitis through an HIF-1-Dependent Mechanism

2019; Cell Press; Volume: 27; Issue: 3 Linguagem: Inglês

10.1016/j.celrep.2019.03.054

2639-1856

José Luís Fachi, Jaqueline de Souza Felipe, Laís Passariello Pral, Bruna Karadi da Silva, Renan Oliveira Corrêa, Mirella Cristiny Pereira de Andrade, Denise Morais da Fonseca, Paulo José Basso, Niels Olsen Saraiva Câmara, Éricka Lorenna de Sales e Souza, Flaviano S. Martins, Suzana Eiko Sato Guima, Andrew Maltez Thomas, João Carlos Setúbal, Yuli Thamires Magalhães, Fábio Luís Forti, Thamiris Candreva, Hosana Gomes Rodrigues, Marcelo Bispo de Jesus, Sílvio Roberto Consonni, Alessandro S. Farias, Patrick Varga‐Weisz, Marco Aurélio Ramirez Vinolo,

Gastrointestinal motility and disorders

Antibiotic-induced dysbiosis is a key factor predisposing intestinal infection by Clostridium difficile. Here, we show that interventions that restore butyrate intestinal levels mitigate clinical and pathological features of C. difficile-induced colitis. Butyrate has no effect on C. difficile colonization or toxin production. However, it attenuates intestinal inflammation and improves intestinal barrier function in infected mice, as shown by reduced intestinal epithelial permeability and bacterial translocation, effects associated with the increased expression of components of intestinal epithelial cell tight junctions. Activation of the transcription factor HIF-1 in intestinal epithelial cells exerts a protective effect in C. difficile-induced colitis, and it is required for butyrate effects. We conclude that butyrate protects intestinal epithelial cells from damage caused by C. difficile toxins via the stabilization of HIF-1, mitigating local inflammatory response and systemic consequences of the infection.