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Proteomic characterisation of leech microglia extracellular vesicles (EVs): comparison between differential ultracentrifugation and Optiprep™ density gradient isolation

2019; Taylor & Francis; Volume: 8; Issue: 1 Linguagem: Inglês

10.1080/20013078.2019.1603048

2001-3078

Tanina Arab, Antonella Raffo‐Romero, Christelle Van Camp, Quentin Lemaire, Françoise Le Marrec‐Croq, Francesco Drago, Soulaimane Aboulouard, Christian Slomianny, A.-S. Lacoste, I Guigon, Hélène Touzet, Michel Salzet, Isabelle Fournier, Christophe Lefebvre, Jacopo Vizioli, P. Sautière,

Lipid Membrane Structure and Behavior

In Mammals, microglial cells are considered as the resident immune cells in central nervous system (CNS). Many studies demonstrated that, after injury, these cells are activated and recruited at the lesion site. Leech microglia present a similar pattern of microglial activation and migration upon experimental lesion of CNS. This activation is associated with the release of a large amount of extracellular vesicles (EVs). We collected EVs released by microglia primary culture and compared two different protocols of isolation: one with differential ultracentrifugation (UC) and one using an additional Optiprep