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Effects of dietary n‐6 polyunsaturated fatty acids on growth performance, body composition, haematological parameters and hepatic physiology of juvenile tiger puffer ( Takifugu rubripes )

2019; Wiley; Volume: 25; Issue: 5 Linguagem: Inglês

10.1111/anu.12924

1365-2095

Houguo Xu, Zhangbin Liao, Qinggong Zhang, Yuliang Wei, Mengqing Liang,

Reproductive biology and impacts on aquatic species

Aquaculture NutritionVolume 25, Issue 5 p. 1073-1086 ORIGINAL ARTICLE Effects of dietary n-6 polyunsaturated fatty acids on growth performance, body composition, haematological parameters and hepatic physiology of juvenile tiger puffer (Takifugu rubripes) Houguo Xu, Houguo Xu orcid.org/0000-0002-1000-9928 Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaSearch for more papers by this authorZhangbin Liao, Zhangbin Liao Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai, ChinaSearch for more papers by this authorQinggong Zhang, Qinggong Zhang Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai, ChinaSearch for more papers by this authorYuliang Wei, Yuliang Wei Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaSearch for more papers by this authorMengqing Liang, Corresponding Author Mengqing Liang liangmq@ysfri.ac.cn orcid.org/0000-0002-3241-414X Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China Correspondence Mengqing Liang, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China. Email: liangmq@ysfri.ac.cnSearch for more papers by this author Houguo Xu, Houguo Xu orcid.org/0000-0002-1000-9928 Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaSearch for more papers by this authorZhangbin Liao, Zhangbin Liao Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai, ChinaSearch for more papers by this authorQinggong Zhang, Qinggong Zhang Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai, ChinaSearch for more papers by this authorYuliang Wei, Yuliang Wei Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaSearch for more papers by this authorMengqing Liang, Corresponding Author Mengqing Liang liangmq@ysfri.ac.cn orcid.org/0000-0002-3241-414X Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China Correspondence Mengqing Liang, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China. Email: liangmq@ysfri.ac.cnSearch for more papers by this author First published: 03 May 2019 https://doi.org/10.1111/anu.12924Citations: 7 Data Availability Statement: Raw reads obtained were deposited at the National Center for Biotechnology Information (NCBI)’s Sequence Read Archive under Accession No. SRP142239 (C and H_ARA in the archived data represents D1 and D6 respectively). Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract A 74-day feeding trial was conducted to investigate the efficacy of n-6 polyunsaturated fatty acid (n-6 PUFA) in diets for tiger puffer. Six experimental diets with graded levels of n-6 PUFA (from 6.01 to 37.98 g/kg) replacing saturated fatty acids (SFAs) were used in this study. Increasing levels of n-6 PUFA resulted in decreased weight gain (from 472.6% to 349.4%), feed efficiency and crude protein content in fish, but increased lipid accumulation in whole fish and liver. Liver samples from fish fed the lowest and highest level of n-6 PUFA were subjected to transcriptomic assay. Analysis of the differentially expressed genes showed that high dietary levels of n-6 PUFA tended to induce glycolysis and triacylglycerol biosynthesis, but reduce synthesis of amino acid and protein, and cause impairment to liver health. The mitochondrial DNA copy number, activity of mitochondrial respiratory chain complex I and glycogen content in the liver, which indicate energy supply status, significantly decreased with increasing levels of dietary n-6 PUFA. In conclusion, high n-6 PUFA/SFA ratios in diet led to reduced energy supply (by approximately 50%) and increased hepatic lipid accumulation (by 55%), which probably consequently negatively affect protein synthesis, liver health and growth of tiger puffer. Open Research DATA ACCESSIBILITY Raw reads obtained were deposited at the National Center for Biotechnology Information (NCBI)’s Sequence Read Archive under Accession No. SRP142239 (C and H_ARA in the archived data represents D1 and D6 respectively). Citing Literature Supporting Information Filename Description anu12924-sup-0001-Supinfo.docxWord document, 279.4 KB Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume25, Issue5October 2019Pages 1073-1086 RelatedInformation