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First Community-Wide, Comparative Cross-Linking Mass Spectrometry Study

2019; American Chemical Society; Volume: 91; Issue: 11 Linguagem: Inglês

10.1021/acs.analchem.9b00658

1520-6882

Claudio Iacobucci, Christine Piotrowski, Ruedi Aebersold, B.C. do Amaral, Philip Andrews, Katja Bernfur, Christoph H. Borchers, Nicolas I. Brodie, James E. Bruce, Yong Cao, Stéphane Chaignepain, Juan D. Chavez, Stéphane Claverol, Jürgen Cox, Trisha N. Davis, Gianluca Degliesposti, Meng‐Qiu Dong, Nufar Edinger, Cecilia Emanuelsson, Marina Gay, Michael Götze, Francisco Gomes‐Neto, Fábio C. Gozzo, Craig Gutierrez, Caroline Haupt, Albert J. R. Heck, Franz Herzog, Lan Huang, Michael R. Hoopmann, Nir Kalisman, Oleg Klykov, Zdeněk Kukačka, Fan Liu, Michael J. MacCoss, Karl Mechtler, Ravit Mesika, Robert L. Moritz, Nagarjuna Nagaraj, Victor Nesati, Ana Gisele C. Neves‐Ferreira, Robert Ninnis, Petr Novák, Francis J. O’Reilly, Matthias Pelzing, Evgeniy V. Petrotchenko, Lolita Piersimoni, Manolo Plasencia, Tara L. Pukala, Kasper D. Rand, Juri Rappsilber, Dana Reichmann, Carolin Sailer, Chris P. Sarnowski, Richard A. Scheltema, Carla Schmidt, David C. Schriemer, Yi Shi, Mark Skehel, Moriya Slavin, Frank Sobott, Victor Solis‐Mezarino, Heike Stephanowitz, Florian Stengel, Christian E. Stieger, Esben Trabjerg, Michael J. Trnka, Marta Vilaseca, Rosa Viner, Yufei Xiang, Şule Yılmaz, Alex Zelter, Daniel S. Ziemianowicz, Alexander Leitner, Andrea Sinz,

Mass Spectrometry Techniques and Applications

The number of publications in the field of chemical cross-linking combined with mass spectrometry (XL-MS) to derive constraints for protein three-dimensional structure modeling and to probe protein-protein interactions has increased during the last years. As the technique is now becoming routine for in vitro and in vivo applications in proteomics and structural biology there is a pressing need to define protocols as well as data analysis and reporting formats. Such consensus formats should become accepted in the field and be shown to lead to reproducible results. This first, community-based harmonization study on XL-MS is based on the results of 32 groups participating worldwide. The aim of this paper is to summarize the status quo of XL-MS and to compare and evaluate existing cross-linking strategies. Our study therefore builds the framework for establishing best practice guidelines to conduct cross-linking experiments, perform data analysis, and define reporting formats with the ultimate goal of assisting scientists to generate accurate and reproducible XL-MS results.