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Gel filtration of ABH blood group substances

1971; Elsevier BV; Volume: 55; Issue: 2 Linguagem: Inglês

10.1016/s0021-9673(01)82559-0

1873-3778

Alberto Pio Fiori, G. Giusti, G. Panari, G Porcelli,

Erythrocyte Function and Pathophysiology

Human saliva from secretors of groups A, B, O and AB was gel filtered on Sephadex G-200 and G-100 columns, and on thin layers and the eluates were tested for A, B and H antigens by the haemagglutination inhibition method. A main excluded fraction (fraction 1) was detected in all the samples examined. A part of this fraction was also excluded from Sepharose 4B. The molecular weight of this fractions can be assumed to be between 2·105 and 5·106. In some samples of saliva, one or two group-specific subfractions were identified (fractions 2 and 3). Fraction 2 is excluded from Sephadex G-50 and probably has a molecular weight between 10 000 and 13 000. Fraction 3 is dialysable and has a lower molecular weight, probably no greater than 1500–2000. Fraction 1 is water-soluble and can be precipitated in alcohol, while fractions 2 and 3 are both water- and alcohol-soluble.