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Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution

2019; Nature Portfolio; Volume: 16; Issue: 6 Linguagem: Inglês

10.1038/s41592-019-0401-3

1548-7105

Bin Yang, Xingye Chen, Yina Wang, Siyu Feng, Veronica Pessino, Nico Stuurman, Nathan Cho, Karen W. Cheng, Samuel J. Lord, Linfeng Xu, Dan Xie, R. Dyche Mullins, Manuel D. Leonetti, Bo Huang,

Photonic and Optical Devices

We designed an epi-illumination SPIM system that uses a single objective and has a sample interface identical to that of an inverted fluorescence microscope with no additional reflection elements. It achieves subcellular resolution and single-molecule sensitivity, and is compatible with common biological sample holders, including multi-well plates. We demonstrated multicolor fast volumetric imaging, single-molecule localization microscopy, parallel imaging of 16 cell lines and parallel recording of cellular responses to perturbations.